ARG52460

anti-beta III Tubulin antibody [AA10]

anti-beta III Tubulin antibody [AA10] for Immunohistochemistry,Western blot and Mouse,Rat

Controls and Markers antibody; Neuroscience antibody; Signaling Transduction antibody; Neuron Development Study antibody; Neuronal Cytoskeletal antibody; Neurite Marker antibody
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Overview

Product Description

Mouse Monoclonal antibody [AA10] recognizes Tubulin, beta III

Tested Reactivity Ms, Rat
Tested Application IHC, WB
Host Mouse
Clonality Monoclonal
Clone AA10
Isotype IgG
Target Name beta III Tubulin
Antigen Species Rat
Immunogen Synthetic peptide corresponding to amino acid residues specific to beta III Tubulin conjugated to KLH
Conjugation Un-conjugated
Alternate Names CDCBM1; Tubulin beta-4 chain; Tubulin beta-3 chain; CFEOM3A; Tubulin beta-III; TUBB4; CDCBM; CFEOM3; FEOM3; beta-4

Application Instructions

Application Suggestion
Tested Application Dilution
IHC1:1000
WB1:1000 - 1:5000
Application Note Specific for the ~55kDa beta III Tubulin protein. This clone is similar to the monoclonal antibody Tuj1.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Protein G purified
Buffer 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol
Stabilizer 0.1 mg/ml BSA, 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 22152 Mouse TUBB3

GeneID: 246118 Rat TUBB3

Swiss-port # Q4QRB4 Rat Tubulin beta-3 chain

Swiss-port # Q9ERD7 Mouse Tubulin beta-3 chain

Gene Symbol TUBB3
Gene Full Name Tubulin, beta 3 class III
Background Tubulin is the major constituent of microtubules, existing as a heterodimer of the α and β subunits. The beta III isoform of Tubulin is found almost exclusively in neuronal processes of adult tissues and is therefore an excellent marker for neurons. Neuron specific, posttranslational modifications within the C-terminal domain of beta III Tubulin have been shown to be developmentally regulated suggesting that they may serve to modulate the interaction of Tubulin with microtubule associated proteins (Lee et al., 1990). Additionally, beta III Tubulin has been found to be highly expressed in cancer cells such as small cell lung cancer, large cell neuroendocrine carcinoma and adenocarcinomas and is correlated with an increasing histological degree of malignancy (Katsetos et al., 2003)
Highlight Related products:
beta III Tubulin antibodies; beta III Tubulin Duos / Panels; Anti-Mouse IgG secondary antibodies;
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Research Area Controls and Markers antibody; Neuroscience antibody; Signaling Transduction antibody; Neuron Development Study antibody; Neuronal Cytoskeletal antibody; Neurite Marker antibody
Calculated MW 50 kDa
PTM Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).
Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable).
Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.

Customer's Feedback

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Review for anti-beta III Tubulin antibody [AA10]

Application:WB

Sample:1) Mouse brain, 2) Rat brain

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC