ARG62683

anti-beta III Tubulin antibody [TU-20]

anti-beta III Tubulin antibody [TU-20] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Western blot and Baboon,Bovine,Broad,Carp,Chicken,Hamster,Human,Mouse,Pig,Rat

Controls and Markers antibody; Neuroscience antibody; Signaling Transduction antibody; Neuron Development Study antibody; Neuronal Cytoskeletal antibody; Neurite Marker antibody
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Overview

Product Description

Mouse Monoclonal antibody [TU-20] recognizes beta III Tubulin

Tested Reactivity Hu, Ms, Rat, Bb, Bov, Broad, Carp, Chk, Hm, Pig
Tested Application FACS, ICC/IF, IHC-Fr, IHC-P, WB
Specificity The clone TU-20 recognizes C-terminal peptide sequence ESESQGPK (aa 441-448) of neuron-specific human beta III Tubulin.
Host Mouse
Clonality Monoclonal
Clone TU-20
Isotype IgG1
Target Name beta III Tubulin
Immunogen Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin via cysteine added to the N-terminus of the neuron-specific peptide.
Conjugation Un-conjugated
Alternate Names CDCBM1; Tubulin beta-4 chain; Tubulin beta-3 chain; CFEOM3A; Tubulin beta-III; TUBB4; CDCBM; CFEOM3; FEOM3; beta-4

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 4 µg/ml
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-P10 µg/ml
WB1 - 2 µg/ml
Application Note WB: In reducing conditions, incubated for 90 min. Positive control: Porcine brain lysate; Negative control: HPB-ALL human peripheral blood leukemia cell line. Sample preparation: Mix lysate with reducing Laemmli SDS-PAGE sample buffer.
IHC-P: Staining technique: Standard ABC technique (DAB+). Antigen Retrieval: Heat the tissue section in 10 mM Citrate buffer (pH 6.0) or pretreat samples with 0.1% pepsin (trypsin) in 0.1M HCl for 30 min at RT.

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Positive tissue: neuronal tissue
Immunocytochemistry: Positive material: Neuro2a mouse neuroblastoma cell line

Properties

Form Liquid
Purification Purified by ammonium sulphate and caprylic acid precipitation
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 10381 Human TUBB3

GeneID: 22152 Mouse TUBB3

GeneID: 246118 Rat TUBB3

Gene Symbol TUBB3
Gene Full Name tubulin, beta 3 class III
Background The beta III-Tubulin isoform is present dominantly in cells of neuronal origin and it is one of the earliest markers of neuronal differentiation. It is regarded as a specific probe for the cells of neuronal origin as well as for the tumours originating from these cells. The betaIII-Tubulin is most abundant in cells of neuronal origin, but was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.
Function Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and mantainance. [UniProt]
Highlight Related Antibody Duos and Panels:
ARG30010 Neuronal Cytoskeletal Antibody Duo (NF-L, TUBBIII)
ARG30301 Neurite Marker Antibody Duo
Related products:
beta III Tubulin antibodies; beta III Tubulin Duos / Panels; Anti-Mouse IgG secondary antibodies;
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Research Area Controls and Markers antibody; Neuroscience antibody; Signaling Transduction antibody; Neuron Development Study antibody; Neuronal Cytoskeletal antibody; Neurite Marker antibody
Calculated MW 50 kDa
PTM Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).
Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable).
Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.

Customer's Feedback

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Review for anti-beta III Tubulin antibody [TU-20]

Application:WB

Sample:1) HeLa (negative control), 2) Mouse brain, 3) Rat brain

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Specific References

BAF45D Downregulation in Spinal Cord Ependymal Cells Following Spinal Cord Injury in Adult Rats and Its Potential Role in the Development of Neuronal Lesions.

WB, IHC-Fr / Rat

Wang Zhenzhen et al.
Front Neurosci.,  (2019)

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