ARG54082

Name: anti-beta Tubulin antibody
Brand: arigo Biolaboratories
SKU: ARG54082
Price: 355 USD
Availability: InStock
Rating: 5.0 (3 reviews)

anti-beta Tubulin antibody

Publication2

Ordering Information

Package

Price (USD)

100 μl

355.00

50 μl

245.00

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Overview

Product Description	Mouse Monoclonal antibody recognizes TUBB
Tested Reactivity	Hu, Ms, Rat, Goat, Hm, Mk
Tested Application	ICC/IF, WB
Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Target Name	beta Tubulin
Antigen Species	Human
Immunogen	Purified recombinant human Tubulin beta protein fragments expressed in E.coli.
Conjugation	Un-conjugated
Alternate Names	OK/SW-cl.56; CDCBM6; Tubulin beta chain; M40; TUBB5; Tubulin beta-5 chain; TUBB1

Application Instructions

Application Suggestion

Tested Application	Dilution
ICC/IF	1:500
WB	1:5000 - 1:20000

Application Note

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form	Liquid
Purification	Affinity purified
Buffer	PBS (pH 7.4), 0.02% Sodium azide, 0.1mg/ml BSA and 50% Glycerol
Preservative	0.02% Sodium azide
Stabilizer	0.1mg/ml BSA, 50% Glycerol
Concentration	1 mg/ml
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 203068 Human TUBB GeneID: 574113 Monkey TUBB Swiss-port # P07437 Human Tubulin beta chain Swiss-port # P69895 Monkey Tubulin beta chain
Gene Symbol	TUBB
Gene Full Name	tubulin, beta class I
Background	Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
Function	Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [UniProt]
Cellular Localization	Cytoplasm, cytoskeleton.
Highlight	Related Antibody Duos and Panels: ARG30270 Loading Control Antibody Panel (Actin, beta Tublin, Histone H3, GAPDH) Related products: beta Tubulin antibodies; beta Tubulin Duos / Panels; Anti-Mouse IgG secondary antibodies;
Research Area	Controls and Markers antibody; Signaling Transduction antibody; Loading Control antibody
Calculated MW	50 kDa
PTM	Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866). Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable). Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.