ARG40573

anti-beta Tubulin antibody (FITC)

anti-beta Tubulin antibody (FITC) for Western blot,ICC/IF and Human,Mouse,Monkey,Goat,Hamster,Rat

Controls and Markers antibody; Signaling Transduction antibody; Loading Control antibody

Overview

Product Description FITC-conjugated Mouse Monoclonal antibody recognizes beta Tubulin
Tested Reactivity Hu, Ms, Rat, Goat, Hm, Mk
Tested Application ICC/IF, WB
Host Mouse
Clonality Monoclonal
Isotype IgG1
Target Name beta Tubulin
Antigen Species Human
Immunogen Human beta Tubulin.
Conjugation FITC
Alternate Names OK/SW-cl.56; CDCBM6; Tubulin beta chain; M40; TUBB5; Tubulin beta-5 chain; TUBB1

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:100
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.4), 0.02% Sodium azide, 50% Glycerol and 1% BSA.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol and 1% BSA
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 203068 Human TUBB

GeneID: 574113 Monkey TUBB

Swiss-port # P07437 Human Tubulin beta chain

Swiss-port # P69895 Monkey Tubulin beta chain

Gene Symbol TUBB
Gene Full Name tubulin, beta class I
Background This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13. [provided by RefSeq, Jun 2014]
Function Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [UniProt]
Cellular Localization Cytoplasm, cytoskeleton. [UniProt]
Research Area Controls and Markers antibody; Signaling Transduction antibody; Loading Control antibody
Calculated MW 50 kDa
PTM Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).

Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable).

Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules. [UniProt]