ARG62684
anti-beta Tubulin antibody [TU-06]
anti-beta Tubulin antibody [TU-06] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Arabidopsis,Chicken,Fish,Human,Mouse,Nicotiana,Paramecium,Pig,Rat,Tetrahymena
Overview
| Product Description | Mouse Monoclonal antibody [TU-06] recognizes beta Tubulin |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Arabi, Chk, Fsh, Nicotiana, Paramecium, Pig, Tetrahymena |
| Tested Application | ICC/IF, IHC-P, WB |
| Specificity | The clone TU-06 recognizes an epitope (aa 81-95) on phylogenetically conserved N-terminal structural domain of beta-Tubulin (recognizes all beta-Tubulin isoforms) in various species. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | TU-06 |
| Isotype | IgM |
| Target Name | beta Tubulin |
| Antigen Species | Pig |
| Immunogen | Beta-subunits of porcine brain Tubulin. |
| Conjugation | Un-conjugated |
| Alternate Names | Tubulin Beta-1 Chain ; Tubulin, Beta 1; DJ543J19.4; Class VI Beta-Tubulin; MACTHC1 |
Application Instructions
| Application Suggestion |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Application Note | WB: Incubated for 60 min. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with reducing Laemmli SDS-PAGE sample buffer. Application note: reducing condition. ICC/IF: Staining technique: Fixed and permeabilized cells. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
||||||||
| Positive Control | WB: HPB-ALL human peripheral blood leukemia cell line IHC-P: Heart ICC/IF: 3T3 |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from ascites by precipitation methods. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | TBS (pH 8.0) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TUBB1 |
| Gene Full Name | Tubulin Beta 1 Class VI |
| Background | This gene encodes a member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is specifically expressed in platelets and megakaryocytes and may be involved in proplatelet production and platelet release. A mutations in this gene is associated with autosomal dominant macrothrombocytopenia. Two pseudogenes of this gene are found on chromosome Y.[provided by RefSeq, Jul 2010] |
| Function | Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin. [Uniprot] |
| Calculated MW | 50 kDa |
| PTM | Isopeptide bond, Phosphoprotein. [Uniprot] |
Images (3) Click the Picture to Zoom In
-
ARG62684 anti-beta Tubulin antibody [TU-06] WB image
Western blot: HPB-ALL human peripheral blood leukemia cell lysate stained with (1) Negative control, (2,3,4,5,6) ARG62684 anti-beta Tubulin antibody [TU-06] (dilutions: 0.5, 1, 2, 4, 5 µg/ml).
-
ARG62684 anti-beta Tubulin antibody [TU-06] IHC-P image
Immunohistochemistry: Paraffin-embedded Human heart tissue stained with ARG62684 anti-beta Tubulin antibody [TU-06].
-
ARG62684 anti-beta Tubulin antibody [TU-06] ICC/IF image
Immunofluorescence: 3T3 mouse embryonal fibroblast cells stained with ARG62684 anti-beta Tubulin antibody [TU-06] (red).
Cell nuclei was stained with DAPI (blue).
