ARG42137

anti-eEF1E1 / AIMP3 antibody

anti-eEF1E1 / AIMP3 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human,Pig

Overview

Product Description Goat Polyclonal antibody recognizes eEF1E1 / AIMP3
Tested Reactivity Hu, Pig
Predict Reactivity Cow, Dog
Tested Application IHC-P, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name eEF1E1 / AIMP3
Antigen Species Human
Immunogen Synthetic peptide around the internal region of Human eEF1E1 / AIMP3. (C-SKGNKYSAQGERQ) (NP_004271.1; NP_001129122.1)
Conjugation Un-conjugated
Alternate Names Eukaryotic translation elongation factor 1 epsilon-1; Elongation factor p18; AIMP3; P18; Multisynthase complex auxiliary component p18; Aminoacyl tRNA synthetase complex-interacting multifunctional protein 3

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P5 µg/ml
WB0.1 - 0.3 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control K562 and Pig testis
Observed Size ~ 20 kDa

Properties

Form Liquid
Purification Ammonium sulphate precipitation followed by affinity purification with immunogen.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 9521 Human EEF1E1

Swiss-port # O43324 Human Eukaryotic translation elongation factor 1 epsilon-1

Gene Symbol EEF1E1
Gene Full Name eukaryotic translation elongation factor 1 epsilon 1
Background This gene encodes a multifunctional protein that localizes to both the cytoplasm and nucleus. In the cytoplasm, the encoded protein is an auxiliary component of the macromolecular aminoacyl-tRNA synthase complex. However, its mouse homolog has been shown to translocate to the nucleus in response to DNA damage, and it plays a positive role in ATM/ATR-mediated p53 activation. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring downstream MUTED (muted homolog) gene. An EEF1E1-related pseudogene has been identified on chromosome 2. [provided by RefSeq, Dec 2010]
Function Positive modulator of ATM response to DNA damage. [UniProt]
Cellular Localization Cytoplasm. Cytoplasm, cytosol. Nucleus. Note=Cytoplasmic under growth arrest conditions. Translocated into the nucleus when growth resumes (S phase) and following DNA damage. [UniProt]
Calculated MW 20 kDa