ARG56163
anti-eIF4E2 antibody
anti-eIF4E2 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes eIF4E2 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | eIF4E2 |
| Antigen Species | Human |
| Immunogen | Recombinant protein of Human eIF4E2. |
| Conjugation | Un-conjugated |
| Alternate Names | Eukaryotic translation initiation factor 4E homologous protein; mRNA cap-binding protein type 3; EIF4EL3; IF4e; Eukaryotic translation initiation factor 4E type 2; 4E-LP; eIF4E-like protein 4E-LP; eIF-4E type 2; mRNA cap-binding protein 4EHP; eIF4E type 2; Eukaryotic translation initiation factor 4E-like 3; 4EHP |
Application Instructions
| Predict Reactivity Note | Rat | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | HL-60 | ||||||||
| Observed Size | ~ 30 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O60573 Human Eukaryotic translation initiation factor 4E type 2 Swiss-port # Q8BMB3 Mouse Eukaryotic translation initiation factor 4E type 2 |
|---|---|
| Gene Symbol | EIF4E2 |
| Gene Full Name | eukaryotic translation initiation factor 4E family member 2 |
| Function | Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. [UniProt] |
| Calculated MW | 28 kDa |
| PTM | Ubiquitinated by ARIH1 (PubMed:14623119, PubMed:25624349). The consequences of ubiquitination are however unclear: according to a report, EIF4E2 ubiquitination leads to promote EIF4E2 cap-binding and protein translation arrest (PubMed:25624349). According to another report ubiquitination leads to its subsequent degradation (PubMed:14623119). ISGylation enhances its cap structure-binding activity and translation-inhibition activity. |
