ARG66856
anti-eNOS antibody
anti-eNOS antibody for IHC-Formalin-fixed paraffin-embedded sections and Human
Overview
| Product Description | Mouse Monoclonal antibody recognizes eNOS |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG |
| Target Name | eNOS |
| Antigen Species | Human |
| Immunogen | Purified recombinant fragment of Human eNOS. |
| Conjugation | Un-conjugated |
| Alternate Names | Constitutive NOS; NOS type III; Nitric oxide synthase, endothelial; Endothelial NOS; eNOS; EC-NOS; NOSIII; cNOS; EC 1.14.13.39; ECNOS |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | Ascitic fluid, 0.03% Sodium azide, 50% Glycerol and 0.5% BSA. |
| Preservative | 0.03% Sodium azide |
| Stabilizer | 50% Glycerol and 0.5% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P29474 Human Nitric oxide synthase, endothelial |
|---|---|
| Gene Symbol | NOS3 |
| Gene Full Name | nitric oxide synthase 3 (endothelial cell) |
| Background | Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Oct 2016] |
| Function | Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. [Isoform eNOS13C]: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1. [UniProt] |
| Cellular Localization | Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Note=Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity. [UniProt] |
| Calculated MW | 133 kDa |
| PTM | Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity (By similarity). Phosphorylation of Ser-114 by CDK5 reduces activity. [UniProt] |
