ARG62989

anti-gamma Tubulin antibody [TU-30]

anti-gamma Tubulin antibody [TU-30] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Bovine,Chicken,Human,Mouse,Pig,Plant,Protozoa,Rat

Signaling Transduction antibody

Overview

Product Description

Mouse Monoclonal antibody [TU-30] recognizes gamma Tubulin

Tested Reactivity Hu, Ms, Rat, Bov, Chk, Pig, Plnt, Prt
Tested Application FACS, ICC/IF, IHC-P, WB
Specificity The clone TU-30 recognizes C-terminal peptide sequence EYHAATRPDYISWGTQ (aa 434-449) of gamma-Tubulin, a 48 kDa structural constituent of cytoskeleton and microtubule organizing center (MTOC). This clone reacts gamma-tubulin 1 but not on human gamma-tubulin 2.
Host Mouse
Clonality Monoclonal
Clone TU-30
Isotype IgG1
Target Name gamma Tubulin
Immunogen C-terminal peptide of gamma-Tubulin counjugated to KLH.
Epitope The epitope was located in the amino acid sequence TRPDYI (aa439-444 in human), which is present on human gamma-tubulin 1 but not on human gamma-tubulin 2.
Conjugation Un-conjugated
Alternate Names GCP-1; CDCBM4; TUBG; Gamma-1-tubulin; Tubulin gamma-1 chain; TUBGCP1; Gamma-tubulin complex component 1

Application Instructions

Application Suggestion
Tested Application Dilution
FACSAssay-dependent
ICC/IF1 - 2 µg/ml
IHC-P1:500 - 1:1000
WB1:500 - 1:2000
Application Note ICC/IF: Staining technique: (a) Fix cells for 10 min in methanol at -20°C and for 6 min in acetone at -20°C; (b) Fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C. Incubation: 45 min at RT. Note: The clone TU-30 stains only fixed cells.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control ICC/IF: P-19 Mouse embryonal carcinoma cell line and 3T3 Mouse fibroblasts.

Properties

Form Liquid
Purification Purified from hybridoma culture supernatant by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 103733 Mouse TUBG1

GeneID: 252921 Rat TUBG1

GeneID: 541101 Bovine TUBG1

Gene Symbol TUBG1
Gene Full Name tubulin, gamma 1
Background The gamma-Tubulin (TUBG1; relative molecular weight about 48 kDa) is a minor member of Tubulin family (less that 0.01% of Tubulin dimer). The gamma-Tubulin ring structures, however, serve to provide structural primer for initiation of microtubular nucleation and growth, thereby being crutial for microtubule-based cellular processes, above all for mitotic spindle formation. In animal cells, a center of microtubule organization is the centrosome composed of a pair of cylindrical centrioles surrounded by fibrous pericentriolar material containing gamma-Tubulin. Formation of the mitotic spindle is preceded by duplication of centrosome during S phase. Before mitosis, both centrosomes increase their microtubule nucleation capacity and form two microtuble asters that are pushed apart from each other by the forces of motor proteins associated at the microtubule surface.
Function Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta chain minus-end nucleation, centrosome duplication and spindle formation. [UniProt]
Highlight Related products:
gamma Tubulin antibodies; Anti-Mouse IgG secondary antibodies;
Related news:
Gene therapy for retinitis pigmentosa (RP)
Related poster download:
Organelle Markers & Loading Control
Research Area Signaling Transduction antibody
Calculated MW 51 kDa
PTM Phosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome.

Clone References

Interactome analysis of the influenza A virus transcription/replication machinery identifies protein phosphatase 6 as a cellular factor required for efficient virus replication.

York A et al.
J Virol.,  (2014)

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Selective impairment of a subset of Ran-GTP-binding domains of ran-binding protein 2 (Ranbp2) suffices to recapitulate the degeneration of the retinal pigment epithelium (RPE) triggered by Ranbp2 ablation.

Patil H et al.
J Biol Chem.,  (2014)

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Mitotic phosphorylation of histone H3 threonine 80.

Hammond SL et al.
Cell Cycle.,  (2014)

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Plus-end tracking proteins, CLASPs, and a viral Akt mimic regulate herpesvirus-induced stable microtubule formation and virus spread.

Naghavi MH et al.
Proc Natl Acad Sci U S A.,  (2013)

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Mitosis phase enrichment with identification of mitotic centromere-associated kinesin as a therapeutic target in castration-resistant prostate cancer.

IHC / Human

Sircar K et al.
PLoS One.,  (2012)

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Kif7 promotes hedgehog signaling in growth plate chondrocytes by restricting the inhibitory function of Sufu.

IHC-P / Mouse

Hsu SH et al.
Development.,  (2011)

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WDR62 is associated with the spindle pole and is mutated in human microcephaly.

IHC-P / Human

Nicholas AK et al.
Nat Genet.,  (2010)

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Discovery of a distinct domain in cyclin A sufficient for centrosomal localization independently of Cdk binding.

ICC/IF / Human, Chinese Hamster, Xenopus laevis

Pascreau G et al.
Proc Natl Acad Sci U S A.,  (2010)

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Dishevelled controls apical docking and planar polarization of basal bodies in ciliated epithelial cells.

IHC-Fr / Xenopus laevis

Park TJ et al.
Nat Genet.,  (2008)

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