ARG62990

anti-gamma Tubulin antibody [TU-32]

anti-gamma Tubulin antibody [TU-32] for ICC/IF,Western blot and Human,Mammal,Mouse,Plant,Protozoa,Rat

Signaling Transduction antibody

Overview

Product Description

Mouse Monoclonal antibody [TU-32] recognizes gamma Tubulin

Tested Reactivity Hu, Ms, Rat, Mamm, Plnt, Prt
Tested Application ICC/IF, WB
Specificity The clone TU-32 recognizes an epitope (amino acids 434-449 in human) within C-terminus of gamma-Tubulin, a 48 kDa structural constituent of cytoskeleton and microtubule organizing center (MTOC).
Host Mouse
Clonality Monoclonal
Clone TU-32
Isotype IgG1
Target Name gamma Tubulin
Antigen Species Human
Immunogen Synthetic peptide around aa. 434-449 of Human gamma Tubulin. (EYHAATRPDYISWGTQ)
Epitope The epitope was located in the aminoacid sequence PDYISW (aa441-446 in human), which is identical for gamma-tubulin 1 and gamma-tubulin 2.
Conjugation Un-conjugated
Alternate Names GCP-1; CDCBM4; TUBG; Gamma-1-tubulin; Tubulin gamma-1 chain; TUBGCP1; Gamma-tubulin complex component 1

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IFAssay-dependent
WB1 µg/ml
Application Note ICC/IF: Methanol/acetone fixation required.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from cell culture supernatant by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 103733 Mouse TUBG1

GeneID: 252921 Rat TUBG1

GeneID: 7283 Human TUBG1

Gene Symbol TUBG1
Gene Full Name tubulin, gamma 1
Background The gamma-Tubulin (TUBG1; relative molecular weight about 48 kDa) is a minor member of Tubulin family (less that 0.01% of Tubulin dimer). The gamma-Tubulin ring structures, however, serve to provide structural primer for initiation of microtubular nucleation and growth, thereby being crutial for microtubule-based cellular processes, above all for mitotic spindle formation. In animal cells, a center of microtubule organization is the centrosome composed of a pair of cylindrical centrioles surrounded by fibrous pericentriolar material containing gamma-Tubulin. Formation of the mitotic spindle is preceded by duplication of centrosome during S phase. Before mitosis, both centrosomes increase their microtubule nucleation capacity and form two microtuble asters that are pushed apart from each other by the forces of motor proteins associated at the microtubule surface.
Function Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta chain minus-end nucleation, centrosome duplication and spindle formation. [UniProt]
Research Area Signaling Transduction antibody
Calculated MW 51 kDa
PTM Phosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome.