ARG58926

anti-hemoglobin alpha antibody

anti-hemoglobin alpha antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes hemoglobin alpha
Tested Reactivity Hu, Ms
Predict Reactivity Mk
Tested Application FACS, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name hemoglobin alpha
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide corresponding to aa. 100-128 of Human hemoglobin alpha.
Conjugation Un-conjugated
Alternate Names HBA-T3; Alpha-globin; HBH; Hemoglobin subunit alpha; Hemoglobin alpha chain

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:25
IHC-P1:50 - 1:100
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Human fetal heart

Properties

Form Liquid
Purification Purification with Protein A and immunogen peptide.
Buffer PBS and 0.09% (W/V) Sodium azide.
Preservative 0.09% (W/V) Sodium azide.
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3039 Human HBA1

Swiss-port # P69905 Human Hemoglobin subunit alpha

Gene Symbol HBA1
Gene Full Name hemoglobin, alpha 1
Background The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin; alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alpha thalassemias have also been reported. [provided by RefSeq, Jul 2008]
Function Involved in oxygen transport from the lung to the various peripheral tissues. [UniProt]
Calculated MW 15 kDa
PTM The initiator Met is not cleaved in variant Thionville and is acetylated. [UniProt]