ARG56509
anti-iNOS antibody
anti-iNOS antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mammal,Mouse,Rat
Inflammation Study antibody; M1/M2/TAM Marker antibody; Macrophage Marker antibody; M1 macrophage Marker antibody
Publication13
Overview
| Product Description | Rabbit Polyclonal antibody recognizes iNOS |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Mamm |
| Tested Application | ICC/IF, IHC-P, IP, WB |
| Specificity | This antibody may cross-react to nNOS (~5%) and does not cross-react to eNOS. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | iNOS |
| Antigen Species | Mouse |
| Immunogen | Purified enzyme from Mouse macrophages (RAW 264.7) cells. |
| Conjugation | Un-conjugated |
| Alternate Names | HEP-NOS; Inducible NO synthase; INOS; Nitric oxide synthase, inducible; iNOS; Hepatocyte NOS; NOS2A; Peptidyl-cysteine S-nitrosylase NOS2; Inducible NOS; NOS; NOS type II; EC 1.14.13.39 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 or Tris-EDTA pH 9.0 for 20 min followed by cooling at RT for 20 min. Incubate with the antibody at 4°C for overnight. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | TBS (pH 7.4) and 0.5 mg/ml BSA. |
| Stabilizer | 0.5 mg/ml BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | Nos2 |
| Gene Full Name | nitric oxide synthase 2, inducible |
| Background | iNOS: Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide synthase is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008] |
| Function | iNOS produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body (PubMed:7531687, PubMed:7544004). In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2. As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM (PubMed:25417112). Involved in inflammation, enhances the synthesis of proinflammatory mediators such as IL6 and IL8 (PubMed:19688109). [UniProt] |
| Highlight | Related Antibody Duos and Panels: ARG30323 Inflammation Antibody Panel ARG30333 M1/M2/TAM Marker Antibody Panel Related products: iNOS antibodies; iNOS ELISA Kits; iNOS Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: New antibody panels and duos for Tumor immune microenvironment Tumor-Infiltrating Lymphocytes (TILs) Exploring Antiviral Immune Response Anti-SerpinB9 therapy, a new strategy for cancer therapy RIP1 activation and pathogenesis of NASH |
| Research Area | Inflammation Study antibody; M1/M2/TAM Marker antibody; Macrophage Marker antibody; M1 macrophage Marker antibody |
| Calculated MW | 131 kDa |
Customer's Feedback
Excellent
Review for anti-iNOS antibody
Application：WB
Sample：Raw264.7 untreated or treated with LPS
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:400
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Specific References
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Evaluation Of The Histopathological, Immunohistochemical, And Oxidative Neurotoxic Effects of Nanoaluminum Present In Human Vaccines In Newborn Albino Rats
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Modulating phenylalanine metabolism by L. acidophilus alleviates alcohol-related liver disease through enhancing intestinal barrier function
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Anti-Inflammatory Actions of G-Protein-Coupled Estrogen Receptor 1 (GPER) and Brain-Derived Estrogen Following Cerebral Ischemia in Ovariectomized Rats
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Paclitaxel induces cognitive impairment via necroptosis, decreased synaptic plasticity and M1 polarisation of microglia
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Berberine ameliorates mesenteric vascular dysfunction by modulating perivascular adipose tissue in diet-induced obese in rats
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Glycolytic Reprogramming in Silica-Induced Lung Macrophages and Silicosis Reversed by Ac-SDKP Treatment
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Baihe Wuyao decoction ameliorates CCl 4-induced chronic liver injury and liver fibrosis in mice through blocking TGF-β1/Smad2/3 signaling, anti-inflammation and anti-oxidation effects.
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A free-standing multilayer film as a novel delivery carrier of platelet lysates for potential wound-dressing applications.
IHC-P / Rat
HYCO-3, a dual CO-releaser/Nrf2 activator, reduces tissue inflammation in mice challenged with lipopolysaccharide.
IHC-P / Mouse
Distribution and proportion of M1/M2 macrophages in periodontal tissues in rats with and without periodontitis
IHC-P / Rat
