ARG53372

anti-iNOS antibody [SP126]

anti-iNOS antibody [SP126] for IHC-Formalin-fixed paraffin-embedded sections and Human

Inflammation Study antibody; M1/M2/TAM Marker antibody; Macrophage Marker antibody; M1 macrophage Marker antibody

Overview

Product Description

Rabbit Monoclonal antibody [SP126] recognizes iNOS

Tested Reactivity Hu
Predict Reactivity Ms, Rat
Tested Application IHC-P
Host Rabbit
Clonality Monoclonal
Clone SP126
Isotype IgG
Target Name iNOS
Antigen Species Human
Immunogen Synthetic peptide near the C-terminus of mouse iNOS protein.
Conjugation Un-conjugated
Alternate Names HEP-NOS; Inducible NO synthase; INOS; Nitric oxide synthase, inducible; iNOS; Hepatocyte NOS; NOS2A; Peptidyl-cysteine S-nitrosylase NOS2; Inducible NOS; NOS; NOS type II; EC 1.14.13.39

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:100
Application Note IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Incubation Time: 10 min at RT.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Lung

Properties

Form Liquid
Purification Purified by protein A/G
Buffer PBS (pH 7.6), 1% BSA and < 0.1% Sodium azide
Preservative < 0.1% Sodium azide
Stabilizer 1% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4843 Human NOS2

Swiss-port # P35228 Human Nitric oxide synthase, inducible

Gene Symbol NOS2
Gene Full Name nitric oxide synthase 2, inducible
Background iNOS: Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide synthase is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]
Function iNOS produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body (PubMed:7531687, PubMed:7544004). In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2. As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM (PubMed:25417112). Involved in inflammation, enhances the synthesis of proinflammatory mediators such as IL6 and IL8 (PubMed:19688109). [UniProt]
Cellular Localization Cytoplasm
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Research Area Inflammation Study antibody; M1/M2/TAM Marker antibody; Macrophage Marker antibody; M1 macrophage Marker antibody
Calculated MW 131 kDa

Clone References

Expression and prognostic significance of iNOS in uveal melanoma.

IHC / Human

Johansson CC et al.
Int J Cancer.,  (2010)

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