ARG54727

Name: anti-mTOR antibody
Brand: arigo Biolaboratories
SKU: ARG54727
Price: 355 USD
Availability: InStock
Rating: 3.5 (2 reviews)

anti-mTOR antibody

Ordering Information

Package

Price (USD)

100 μl

355.00

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Datasheet
Review (2)

Overview

Product Description	Rabbit Polyclonal antibody recognizes mTOR
Tested Reactivity	Hu
Predict Reactivity	Ms, Rat
Tested Application	ICC/IF, IHC-P, WB
Host	Rabbit
Clonality	Polyclonal
Target Name	mTOR
Antigen Species	Human
Immunogen	KLH-conjugated synthetic peptide corresponding to aa. 2459-2488 of Human mTOR (NP_004949.1).
Conjugation	Un-conjugated
Alternate Names	Mammalian target of rapamycin; RAFT1; Mechanistic target of rapamycin; Rapamycin target protein 1; FRAP1; FRAP2; Rapamycin and FKBP12 target 1; FK506-binding protein 12-rapamycin complex-associated protein 1; mTOR; RAPT1; FKBP12-rapamycin complex-associated protein; FRAP; Serine/threonine-protein kinase mTOR; EC 2.7.11.1

Application Instructions

Application Suggestion

Tested Application	Dilution
ICC/IF	1:10 - 1:50
IHC-P	Assay-dependent
WB	1:500 - 1:1000

Application Note

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Purification	Protein A purified
Buffer	PBS and 0.09% (W/V) Sodium azide
Preservative	0.09% (W/V) Sodium azide
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 2475 Human MTOR Swiss-port # P42345 Human Serine/threonine-protein kinase mTOR
Gene Symbol	MTOR
Gene Full Name	mechanistic target of rapamycin (serine/threonine kinase)
Background	The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq, Sep 2008]
Function	Serine/threonine protein kinase which is a central regulator of cellular metabolism, growth and survival in response to hormones, growth factors, nutrients, energy and stress signals. MTOR directly or indirectly regulates the phosphorylation of at least 800 proteins. [From Uniprot]
Cellular Localization	Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side. Lysosome Cytoplasm. Nucleus, PML body Note=Shuttles between cytoplasm and nucleus. Accumulates in the nucleus in response to hypoxia (By similarity). Targeting to lysosomes depends on amino acid availability and RRAGA and RRAGB
Highlight	Related products: mTOR antibodies; mTOR Duos / Panels; Related news: Baking soda restores circadian clock in tumor cells
Research Area	Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Metabolism antibody
Calculated MW	289 kDa
PTM	Autophosphorylates when part of mTORC1 or mTORC2. Phosphorylation at Ser-1261, Ser-2159 and Thr-2164 promotes autophosphorylation. Phosphorylation in the kinase domain modulates the interactions of MTOR with RPTOR and PRAS40 and leads to increased intrinsic mTORC1 kinase activity. Phosphorylation at Thr-2173 in the ATP-binding region by AKT1 strongly reduces kinase activity.