ARG10582
anti-p60 antibody [E17-L]
anti-p60 antibody [E17-L] for IHC-Formalin-fixed paraffin-embedded sections and Human
Overview
| Product Description | Rabbit Monoclonal antibody [E17-L] recognizes p60 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | E17-L |
| Isotype | IgG |
| Target Name | p60 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around the C-terminus of Human p60 |
| Conjugation | Un-conjugated |
| Alternate Names | CAF1A; CAF-IP60; CAF-I 60 kDa subunit; MPP7; MPHOSPH7; CAF1; CAF-1; Chromatin assembly factor I p60 subunit; M-phase phosphoprotein 7; CAF1P60; Chromatin assembly factor 1 subunit B; CAF-1 subunit B; CAF-I p60 |
Application Instructions
| Application Suggestion |
|
||||
|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Buffer | 20 mM Tris-HCl (pH 8.0), 0.05% Sodium azide and 20 mg/ml BSA |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 20 mg/ml BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q13112 Human Chromatin assembly factor 1 subunit B |
|---|---|
| Gene Symbol | CHAF1B |
| Gene Full Name | chromatin assembly factor 1, subunit B (p60) |
| Background | Chromatin assembly factor I (CAF-I) is required for the assembly of histone octamers onto newly-replicated DNA. CAF-I is composed of three protein subunits, p50, p60, and p150. The protein encoded by this gene corresponds to the p60 subunit and is required for chromatin assembly after replication. The encoded protein is differentially phosphorylated in a cell cycle-dependent manner. In addition, it is normally found in the nucleus except during mitosis, when it is released into the cytoplasm. This protein is a member of the WD-repeat HIR1 family and may also be involved in DNA repair. [provided by RefSeq, Jul 2008] |
| Function | Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. [UniProt] |
| Calculated MW | 61 kDa |
| PTM | Differentially phosphorylated during cell cycle. During mitosis the p60 subunit of inactive CAF-1 is hyperphosphorylated and displaced into the cytosol. Progressivly dephosphorylated from G1 to S and G2 phase. Phosphorylated p60 is recruited to chromatin undergoing DNA repair after UV irradiation in G1, S or G2 phases. |
