ARG63503
anti-p70 S6 Kinase antibody
anti-p70 S6 Kinase antibody for Western blot and Human,Mouse
Cell Biology and Cellular Response antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes p70 S6 Kinase |
|---|---|
| Tested Reactivity | Hu, Ms |
| Predict Reactivity | Cow, Rat, Dog |
| Tested Application | WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | p70 S6 Kinase |
| Antigen Species | Human |
| Immunogen | C-MISKRPEHLRMNL |
| Conjugation | Un-conjugated |
| Alternate Names | p70 S6 kinase alpha; p70(S6K)-alpha; p70-alpha; p70 S6KA; S6K; STK14A; S6K1; p70-S6K 1; Ribosomal protein S6 kinase I; 70 kDa ribosomal protein S6 kinase 1; S6K-beta-1; P70S6K1; PS6K; p70 ribosomal S6 kinase alpha; p70-S6K; EC 2.7.11.1; Serine/threonine-protein kinase 14A; p70 S6K-alpha; Ribosomal protein S6 kinase beta-1 |
Application Instructions
| Application Suggestion |
|
||||
|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P23443 Human Ribosomal protein S6 kinase beta-1 Swiss-port # Q8BSK8 Mouse Ribosomal protein S6 kinase beta-1 |
|---|---|
| Background | This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein. The kinase activity of this protein leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding this gene and overexpression of this kinase are seen in some breast cancer cell lines. Alternate translational start sites have been described and alternate transcriptional splice variants have been observed but have not been thoroughly characterized. [provided by RefSeq, Jul 2008] |
| Research Area | Cell Biology and Cellular Response antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 59 kDa |
| PTM | Phosphorylation at Thr-412 is regulated by mTORC1. The phosphorylation at this site is maintained by an agonist-dependent autophosphorylation mechanism (By similarity). Activated by phosphorylation at Thr-252 by PDPK1. Dephosphorylation by PPP1CC at Thr-412 in mitochondrion. |
