ARG42827
anti-p70 S6 Kinase beta phospho (Ser371) antibody
anti-p70 S6 Kinase beta phospho (Ser371) antibody for Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes p70 S6 Kinase beta phospho (Ser371) |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | p70 S6 Kinase beta |
| Antigen Species | Human |
| Immunogen | Phosphospecific peptide around Ser371 of Human p70 S6 Kinase beta. |
| Conjugation | Un-conjugated |
| Alternate Names | S6K-beta2; p70 ribosomal S6 kinase beta; Ribosomal protein S6 kinase beta-2; S6 kinase-related kinase; S6K-beta-2; p70-beta; p70 S6K-beta; p70 S6 kinase beta; p70S6Kb; STK14B; p70 S6KB; p70-S6K 2; P70-beta-1; P70-beta-2; S6K2; SRK; P70S6K2; KLS; Serine/threonine-protein kinase 14B; S6K-beta; P70-beta; p70(S6K)-beta; 70 kDa ribosomal protein S6 kinase 2; EC 2.7.11.1 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Positive Control | K562 | ||||
| Observed Size | ~ 65 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | 50 nM Tris-Glycine (pH 7.4), 0.15 M NaCl, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 40% Glycerol and 0.05% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9UBS0 Human Ribosomal protein S6 kinase beta-2 Swiss-port # Q9Z1M4 Mouse Ribosomal protein S6 kinase beta-2 |
|---|---|
| Gene Symbol | RPS6KB2 |
| Gene Full Name | ribosomal protein S6 kinase, 70kDa, polypeptide 2 |
| Background | This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains a kinase catalytic domain and phosphorylates the S6 ribosomal protein and eukaryotic translation initiation factor 4B (eIF4B). Phosphorylation of S6 leads to an increase in protein synthesis and cell proliferation. [provided by RefSeq, Jan 2015] |
| Function | Phosphorylates specifically ribosomal protein S6 (PubMed:29750193). Seems to act downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression in an alternative pathway regulated by MEAK7 (PubMed:29750193). [UniProt] |
| Cellular Localization | Cytoplasm. Nucleus. [UniProt] |
| Calculated MW | 53 kDa |
| PTM | Phosphorylated and activated by MTOR. Phosphorylation by PKC within the NLS in response to mitogenic stimuli causes cytoplasmic retention. [UniProt] |
