ARG10045
anti-serum Amyloid A antibody [115]
anti-serum Amyloid A antibody [115] for ELISA,Western blot and Human
Cell Biology and Cellular Response antibody; Metabolism antibody
Overview
| Product Description | Mouse Monoclonal antibody [115] recognizes serum Amyloid A |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, WB |
| Specificity | Does not show any cross-reaction with other human cytokines or growth factors tested such as IL-1β, IL-8, MCAF, TGF-β and EGF. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 115 |
| Isotype | IgG1, kappa |
| Target Name | serum Amyloid A |
| Antigen Species | Human |
| Immunogen | Highly purified recombinant human serum Amyloid A (MW: 12 kDa) |
| Conjugation | Un-conjugated |
| Alternate Names | 2-104; 2-103; 2-102; Serum amyloid A-1 protein; TP53I4; SAA; Amyloid fibril protein AA; SAA2; PIG4; 4-101; 3-104 |
Application Instructions
| Application Note | ELISA: This antibody can be used as a capture antibody in sandwich ELISA applications for human SAA detection in combination with a monoclonal tracer/detection antibody (HRP-conjugated, Cat. No.: ARG10205). Suggested capture coating dose: 0.3μg/well. Western Blot: The antibody, when used at concentration of 0.1-0.5μg/mL will allow visualization of 100 ng/lane of recombinant human SAA. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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Properties
| Form | Liquid |
|---|---|
| Purification | Protein G affinity purified |
| Buffer | 0.01M PBS (pH 7.2) |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SAA1 |
| Gene Full Name | serum amyloid A1 |
| Background | This gene encodes a member of the serum amyloid A family of apolipoproteins. The encoded protein is a major acute phase protein that is highly expressed in response to inflammation and tissue injury. This protein also plays an important role in HDL metabolism and cholesterol homeostasis. High levels of this protein are associated with chronic inflammatory diseases including atherosclerosis, rheumatoid arthritis, Alzheimer's disease and Crohn's disease. This protein may also be a potential biomarker for certain tumors. Alternate splicing results in multiple transcript variants that encode the same protein. A pseudogene of this gene is found on chromosome 11.[provided by RefSeq, Jun 2012] |
| Function | Major acute phase protein. [UniProt] |
| Highlight | Related Antibody Duos and Panels: ARG30077 serum Amyloid A ELISA Antibody Duo Related products: serum Amyloid A antibodies; serum Amyloid A ELISA Kits; serum Amyloid A Duos / Panels; Anti-Mouse IgG secondary antibodies; |
| Research Area | Cell Biology and Cellular Response antibody; Metabolism antibody |
| Calculated MW | 14 kDa |
| PTM | This protein is the precursor of amyloid protein A, which is formed by the removal of approximately 24 residues from the C-terminal end. |
Clone References
Comparative proteomic analysis in children with idiopathic short stature (ISS) before and after short-term recombinant human growth hormone (rhGH) therapy.
Elevation of intact and proteolytic fragments of acute phase proteins constitutes the earliest systemic antiviral response in HIV-1 infection.
WB / Human
Serum amyloid A inhibits apoptosis of human neutrophils via a P2X7-sensitive pathway independent of formyl peptide receptor-like 1.
Inflammatory protein profile during systemic high dose interleukin-2 administration.
