ARG55724

anti-tdTomato antibody

anti-tdTomato antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Western blot and Other

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Overview

Product Description

Goat Polyclonal antibody recognizes tdTomato

Tested Reactivity Other
Tested Application ICC/IF, IHC-Fr, IHC-P, WB
Specificity This antibody recognizes tdTomato, mCherry, mApple and red fluorescent protein (DsRed). It does not cross-react to GFP.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name tdTomato
Immunogen Purified full-length recombinant protein produced in E. coli.
Conjugation Un-conjugated
Alternate Names Cherry fluorescent protein; DsRed; mCherry; red fluorescent protein; RFP

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50-1:500
IHC-Fr1:50-1:500
IHC-P1:50-1:500
WB1:500-1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS, 0.05% Sodium azide and 20% Glycerol
Preservative 0.05% Sodium azide
Stabilizer 20% Glycerol
Concentration 3 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Background tdTomato protein is derived from DsRed, an engineered red fluorescent protein from so-called disc corals of the genus Discosoma. It is a genetic fusion of two copies of the dTomato gene, which has been specifically designed for low aggregation. It's brightness and emission wavelength makes it ideal for live animal research.

Specific References

GCNA is a histone binding protein required for spermatogonial stem cell maintenance

IHC-P / Mouse

Jonathan Ribeiro et al.
Nucleic Acids Res.,  (2023)

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Molecular Disambiguation of Heart Rate Control by the Nucleus Ambiguus

IHC-Fr / Mouse

Maira Jalil et al.
bioRxiv.,  (2023)

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Leptin receptor neurons in the dorsomedial hypothalamus input to the circadian feeding network

IHC-Fr / Mouse

Qijun Tang et al.
Sci Adv.,  (2023)

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BMSCs Promote Differentiation of Enteric Neural Precursor Cells to Maintain Neuronal Homeostasis in Mice With Enteric Nerve Injury

ICC/IF, IHC-free-floating / Mouse

Mengke Fan et al.
Cell Mol Gastroenterol Hepatol,  (2022)

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