ARG62485
anti-vWF antibody [F8/86]
anti-vWF antibody [F8/86] for IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody
Publication1
Overview
| Product Description | Mouse Monoclonal antibody [F8/86] recognizes vWF |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Rb |
| Tested Application | IHC-Fr, IHC-P |
| Specificity | This antibody reacts with Von Willebrand Factor in endothelial cells. It also reacts with megakaryocytes in human bone marrow. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | F8/86 |
| Isotype | IgG1 |
| Target Name | vWF |
| Antigen Species | Human |
| Immunogen | Von Willebrand Factor isolated from human plasma. |
| Conjugation | Un-conjugated |
| Alternate Names | VWD; von Willebrand factor; vWF; von Willebrand antigen II; F8VWF |
Application Instructions
| Application Suggestion |
|
||||||
|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: 1. Protease digestion method. (PMID: 9764855) 2. HIER Citrate buffer (pH 6.0) or EDTA (pH 8.0) * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Buffer | Tissue culture supernatant, 0.05% Sodium azide and 1% BSA |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 1% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | VWF |
| Gene Full Name | von Willebrand factor |
| Background | The glycoprotein encoded by this gene functions as both an antihemophilic factor carrier and a platelet-vessel wall mediator in the blood coagulation system. It is crucial to the hemostasis process. Mutations in this gene or deficiencies in this protein result in von Willebrand's disease. An unprocessed pseudogene has been found on chromosome 22. [provided by RefSeq, Jul 2008] |
| Function | Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma. [UniProt] |
| Cellular Localization | Secreted |
| Highlight | VWF (Von Willebrand Factor) is a Protein Coding gene. Diseases associated with VWF include von willebrand's disease and von willebrand disease, type 1. Among its related pathways are PI3K-Akt signaling pathway and Signaling by GPCR. GO annotations related to this gene include protein homodimerization activity and protein N-terminus binding. An important paralog of this gene is OTOGL. [Supplied by GeneCards] |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody |
| Calculated MW | 309 kDa |
| PTM | All cysteine residues are involved in intrachain or interchain disulfide bonds. N- and O-glycosylated. |
Specific References
Pathobiological Study of Leiomyomatoid Angiomatous Neuroendocrine Tumor (LANT)-Like Tumor in the Myometrium.
IHC-P / Human
