ARG83520

arigoQIKTM Human GM-CSF ELISA Kit

arigoQIKTM Human GM-CSF ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG83520-01 Antibody Coated Microplate 8 X 12 strips 4°C
ARG83520-02 Standard 2 vials 4°C
ARG83520-03 1000X HRP-Streptavidin solution 15 μl 4°C
ARG83520-04 Standard/Sample Diluent Buffer 20 ml 4°C
ARG83520-05 HRP Diluent Buffer 20 ml 4°C
ARG83520-06 10X Wash Buffer 35 ml 4°C
ARG83520-07 TMB substrate 12 ml 4°C (protect from light)
ARG83520-08 STOP solution 12 ml 4°C
ARG83520-09 Plate sealer 2 strips Room temperature

Overview

Product Description ARG83520 arigoQIKTM Human GM-CSF ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human GM-CSF in serum, plasma and cell culture supernatants.

Tested Reactivity Hu
Tested Application ELISA
Target Name GM-CSF
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 3.9 pg/mL
Sample Type Serum, plasma and cell culture supernatants.
Standard Range 7.81 - 500 pg/mL
Sample Volume 50 μL
Precision Intra-Assay CV: less than 10%
Inter-Assay CV: less than 10%
Alternate Names CSF2; Colony Stimulating Factor; GMCSF; Sargramostim; GM-CSF; Colony Stimulating Factor 2 (Granulocyte-Macrophage); Granulocyte-Macrophage Colony-Stimulating Factor; Molgramostim; Molgramostin; CSF; Granulocyte-Macrophage Colony Stimulating Factor; Granulocyte Macrophage-Colony Stimulating Factor; Colony-Stimulating Factor

Application Instructions

Assay Time 2 hours

Properties

Form 96 well
Storage Instruction Store the kit at 4°C, -20°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1437 Human CSF2

Swiss-port # P04141 Human Granulocyte-macrophage colony-stimulating factor

Gene Symbol CSF2
Gene Full Name Colony Stimulating Factor 2
Background The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. This gene plays a role in promoting tissue inflammation. Elevated levels of cytokines, including the one produced by this gene, have been detected in SARS-CoV-2 infected patients that develop acute respiratory distress syndrome. Mice deficient in this gene or its receptor develop pulmonary alveolar proteinosis. [provided by RefSeq, Aug 2020]
Function Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. [Uniprot]
Cellular Localization Secreted. [Uniprot]
Title Download Link
ARG83520 arigoQIKTM Human GM-CSF ELISA Kit User manual download icon Download