Product Highlight

In multicellular organisms, cells no longer needed or have become a threat to the organism will be destroyed by a tightly regulated suicide mechanism called programmed cell death, or more commonly known as Apoptosis.

Apoptosis process involves a cascade of proteolytic cleavage of Caspase molecules. Apoptosis can be initiated by intra- or extracellular death signals and the regulation of apoptosis can involve a wide variety of molecules.

arigo offers Antibody Duos for the study of key stages in Apoptosis regulation.

Product name: Apoptosis Marker Antibody Duo (Caspase 3, PARP)
Cat no: ARG30105

The TUNEL assay has been commonly used as a detection method for identifying apoptotic cells. While useful, it can also yield highly variable results as some non-apoptotic nuclei showing high levels of RNA synthesis/splicing can also be labeled by TUNEL technique.
Cleavage of Caspase 3 and PARP (Poly (ADP-ribose) Polymerase is a pre-requisite for cells who undergo final stage of cell death. The use of anti-cleaved caspase3 (ARG20003) and anti-cleaved PARP (ARG20041) antibodies adds a more specific weapon to the arsenal of techniques for confirming apoptosis in tissue specimens.

Constitutively active GSK-3 acts to destabilize beta catenin through phosphorylation (Ser37), thus, functioning as a tumor suppressor to limit proliferation. In contrast, GSK-3 promotes expansion of leukemic cells transformed by HOX overexpression through phosphorylation of CREB (Ser133) and subsequent formation of a transcriptional coactivator complex. (Cancer Cell 17, June 15, 2010)

arigo provides CREB / beta Catenin phospho Duos, ARG30024, which include beta catenin (phosphor Ser37) antibody and CREB (phosphor Ser133) antibody for researchers to analysis the downstream protein and the pathway of active-GSK-3

Component:

ARG51556 CREB(pSer133)Antibody

ARG51710 β Catenin (pSer37) Antibody