ARG23757
Goat anti-Mouse IgG (H+L) antibody (FITC), pre-adsorbed
Goat anti-Mouse IgG (H+L) antibody (FITC), pre-adsorbed for ELISA,ELISPOT,Flow cytometry,FLISA,ICC/IF,IHC-Frozen sections,Western blot and Mouse
Overview
| Product Description | FITC-conjugated Goat Polyclonal antibody recognizes Mouse IgG (H+L) |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA, ELISPOT, FACS, FLISA, ICC/IF, IHC-Fr, WB |
| Specificity | Reacts with the heavy and light chains of mouse IgG1, IgG2a, IgG2b, and IgG3, and with the light chains of mouse IgM and IgA as demonstrated by ELISA, FLISA, and/or flow cytometry; minimal cross reactivity with human immunoglobulins |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | IgG (H+L) |
| Antigen Species | Mouse |
| Conjugation | FITC |
Application Instructions
| Pre Adsorbed | Human immunoglobulins and pooled sera. | ||||||||||||||||
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| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS and 0.1% Sodium azide. |
| Preservative | 0.1% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Images (4) Click the Picture to Zoom In
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ARG23757 Goat anti-Mouse IgG (H+L) antibody (FITC), pre-adsorbed ICC/IF image
Immunofluorescence: Activated Human neutrophils of AAV patients were stained with ARG23757 Goat anti-Mouse IgG (H+L) antibody (FITC), pre-adsorbed and DAPI.
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ARG23757 Goat anti-Mouse IgG (H+L) antibody (FITC), pre-adsorbed FACS image
Flow Cytometry: Human peripheral blood lymphocytes were stained with Mouse anti-Human CD3 followed by ARG23757 Goat anti-Mouse IgG (H+L) antibody (FITC), pre-adsorbed.
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ARG23757 Goat anti-Mouse IgG (H+L) antibody (FITC), pre-adsorbed IHC-P image
Immunohistochemistry: Paraffin-embedded Human kidney section of AAV patients were stained with ARG23757 Goat anti-Mouse IgG (H+L) antibody (FITC), pre-adsorbed and DAPI.
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ARG23757 Goat anti-Mouse IgG (H+L) antibody (FITC), pre-adsorbed ICC/IF image
Immunofluorescence: Human epithelial carcinoma cell line HEp-2 was stained with Mouse anti-Human CD44 (right) followed by ARG23757 Goat anti-Mouse IgG (H+L) antibody (FITC), pre-adsorbed and DAPI.
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Review for Goat anti-Mouse IgG (H+L) antibody (FITC), pre-adsorbed
Application：IF/ICC
Sample：Rabbit skeletal muscle
Fixation Buffer：4% PFA
Fixation Time：20 min
Fixation Temperature：25 ºC
Antigen Retrieval Buffer：-
Primary Antibody Dilution Factor：1 : 100
Primary Antibody Incubation Time：Overnight
Primary Antibody Incubation Temperature：4 ºC
