ARG63121
anti-5-bromodeoxyuridine / BrdU antibody [MoBu-1]
anti-5-bromodeoxyuridine / BrdU antibody [MoBu-1] for Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections and Other
Cell Biology and Cellular Response antibody; Controls and Markers antibody; Gene Regulation antibody; Neuroscience antibody
Publication2
Overview
| Product Description | Mouse Monoclonal antibody [MoBu-1] recognizes 5-bromodeoxyuridine / BrdU |
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| Tested Reactivity | Other |
| Tested Application | FACS, ICC/IF, IHC-Fr, IHC-P |
| Specificity | The clone MoBu-1 reacts specifically with BrdU incorporated into DNA during S-phase of a cell cycle. MoBu-1 is also useful for detecting proliferating cells by flow cytometry or immunofluorescence staining. It reacts also specifically with 5-bromouridine (BrU). The clone does not cross-react to EdU (PMID: 23272138). |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | MoBu-1 |
| Isotype | IgG1 |
| Target Name | 5-bromodeoxyuridine / BrdU |
| Immunogen | 5-bromodeoxyuridine conjugated with hemocyanine. |
| Conjugation | Un-conjugated |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from ascites by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Background | Bromodexyuridine (BrdU) is a thymidine analog which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU. |
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| Highlight | Related products: BrdU antibodies; BrdU Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: Neuronal Development Marker Choose the Best ZIKA Virus Antibodies Fight microcephaly with arigo |
| Research Area | Cell Biology and Cellular Response antibody; Controls and Markers antibody; Gene Regulation antibody; Neuroscience antibody |
Images (2) Click the Picture to Zoom In
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ARG63121 anti-5-bromodeoxyuridine / BrdU antibody [MoBu-1] FACS image
Flow Cytometry: 5-bromodeoxyuridin (BrdU) incorporated CEM cells stained with ARG63121 anti-5-bromodeoxyuridine / BrdU antibody [MoBu-1], followed by incubation with FITC-labelled secondary antibody.
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ARG63121 anti-5-bromodeoxyuridine / BrdU antibody [MoBu-1] IHC-P image
Immunohistochemistry: Bromodeoxyuridine-labeled cells (chick embryo; paraffin-embedded sections) stained with ARG63121 anti-5-bromodeoxyuridine / BrdU antibody [MoBu-1].
Specific References
Vascular Endothelial Cell-derived Exosomes Protect Neural Stem Cells Against Ischemia/reperfusion Injury.
IHC-Fr / Other
Activation of AMPK pathway compromises Rab11 downregulation-mediated inhibition of Schwann cell proliferation in a Glut1 and Glut3-dependent manner.
ICC/IF / Other
