ARG62337
anti-6X His tag antibody [HIS.H8]
anti-6X His tag antibody [HIS.H8] for Dot blot,Electrophoretic mobility shift assay,ELISA,ICC/IF,Immunoprecipitation,Western blot and Other
Controls and Markers antibody; Tag Internal Control antibody
Overview
| Product Description | Mouse Monoclonal antibody [HIS.H8] recognizes 6X His tag |
|---|---|
| Tested Reactivity | Other |
| Tested Application | Dot, ELISA, EMSA, ICC/IF, IP, WB |
| Specificity | Recognizes the epitope of 6x His-tags encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences (i.e.: reacts with N-terminal, C-terminal or internal 6X His-tags) |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | HIS.H8 |
| Isotype | IgG2b |
| Target Name | 6X His tag |
| Antigen Species | Others |
| Immunogen | KLH-conjugated HHHHHH (6X His) synthetic peptide. |
| Conjugation | Un-conjugated |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Protein A Purified |
| Purification Note | Protein A affinity chromatography from mouse ascites fluid. |
| Buffer | 10mM PBS (pH 7.2) and 0.05% Sodium azide. |
| Preservative | 0.05% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Background | The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. His-tagged protein is also useful for the scientitists for the binding assay and tracing the location of the protein-interested in the cells when the specific antibodies for the protein-of-interest are unavailable. |
|---|---|
| Highlight | Related Antibody Duos and Panels: ARG30002 Tag Internal Control Antibody Duo (His tag, GAPDH) Related products: 6x His tag antibodies; 6x His tag ELISA Kits; 6x His tag Duos / Panels; Anti-Mouse IgG secondary antibodies; |
| Research Area | Controls and Markers antibody; Tag Internal Control antibody |
Images (3) Click the Picture to Zoom In
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ARG62337 anti-6X His tag antibody [HIS.H8] ICC/IF image
TOP: Untransfected control; BOTTOM: ARG62337 anti-6X His tag antibody [HIS.H8] (red) on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (blue).
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ARG62337 anti-6X His tag antibody [HIS.H8] WB image
Western blot: (1) untransfected control, (2) HEK293 cells transfected with His-tagged protein vector stained with ARG62337 anti-6X His tag antibody [HIS.H8].
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ARG62337 anti-6X His tag antibody [HIS.H8] WB image
Western blot: A standard ladder containing five different His-tagged proteins stained with ARG62337 anti-6X His tag antibody [HIS.H8] at 1:2000 (0.5 μg/ml) to compare between.
Customer's Feedback
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Review for anti-6X His tag antibody [HIS.H8]
Application：WB
Sample：The crude BL21 soluble fractions containing recombinant PA28α, PA28β, and PA28γ proteasome subunits
Sample Loading Amount：1 µg
Primary Antibody Dilution Factor：1:10000
Primary Antibody Incubation Time：1 hour
Primary Antibody Incubation Temperature：25 ºC
Clone References
Sentrin/SUMO specific proteases as novel tissue-selective modulators of vitamin D receptor-mediated signaling.
WB /
Protein interaction screening for the ankyrin repeats and suppressor of cytokine signaling (SOCS) box (ASB) family identify Asb11 as a novel endoplasmic reticulum resident ubiquitin ligase.
WB /
Binding of G-quadruplexes to the N-terminal recognition domain of the RNA helicase associated with AU-rich element (RHAU).
IP /
Smad6 inhibits non-canonical TGF-β1 signalling by recruiting the deubiquitinase A20 to TRAF6.
A newly uncovered group of distantly related lysine methyltransferases preferentially interact with molecular chaperones to regulate their activity.
WB / Human
The embryonic transcription cofactor LBH is a direct target of the Wnt signaling pathway in epithelial development and in aggressive basal subtype breast cancers.
EMSA /
Subcellular localization of CIAPIN1.
ICC/IF /
