ARG59212

anti-CCDC6 antibody

anti-CCDC6 antibody for Flow cytometry,ICC/IF,Western blot and Human,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes CCDC6
Tested Reactivity Hu, Rat
Predict Reactivity Bov, Chk, Dog, Hm, Hrs, Mk, Rb, Zfsh
Tested Application FACS, ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CCDC6
Antigen Species Human
Immunogen Synthetic peptide corresponding to aa. 156-198 of Human CCDC6. (KAELEQHLEQEQEFQVNKLMKKIKKLENDTISKQLTLEQLRRE)
Conjugation Un-conjugated
Alternate Names TPC; PTC; D10S170; H4; Papillary thyroid carcinoma-encoded protein; Coiled-coil domain-containing protein 6; Protein H4; TST1

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:150 - 1:500
ICC/IF1:200 - 1:1000
WB0.1 - 0.5 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA.
Preservative 0.05% Sodium azide
Stabilizer 5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 8030 Human CCDC6

Swiss-port # Q16204 Human Coiled-coil domain-containing protein 6

Gene Symbol CCDC6
Gene Full Name coiled-coil domain containing 6
Background This gene encodes a coiled-coil domain-containing protein. The encoded protein is ubiquitously expressed and may function as a tumor suppressor. A chromosomal rearrangement resulting in the expression of a fusion gene containing a portion of this gene and the intracellular kinase-encoding domain of the ret proto-oncogene is the cause of thyroid papillary carcinoma.[provided by RefSeq, Sep 2010]
Cellular Localization Cytoplasm. Cytoplasm, cytoskeleton. Note=May be a cytoskeletal protein. [UniProt]
Calculated MW 53 kDa

Images (3) Click the Picture to Zoom In

  • ARG59212 anti-CCDC6 antibody ICC/IF image

    Immunofluorescence: T-47D cells were blocked with 10% goat serum and then stained with ARG59212 anti-CCDC6 antibody (green) at 5 µg/ml dilution, overnight at 4°C. DAPI (blue) for nuclear staining.

  • ARG59212 anti-CCDC6 antibody WB image

    Western blot: Rat testis and MCF-7 whole cell lysate stained with ARG59212 anti-CCDC6 antibody at 0.5 µg/ml dilution.

  • ARG59212 anti-CCDC6 antibody FACS image

    Flow Cytometry: Caco-2 cells were blocked with 10% normal goat serum and then stained with ARG59212 anti-CCDC6 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.