ARG63918

anti-CCKBR antibody

anti-CCKBR antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Metabolism antibody; Neuroscience antibody

Overview

Product Description Goat Polyclonal antibody recognizes CCKBR
Tested Reactivity Hu, Ms, Rat
Predict Reactivity Cow
Tested Application IHC-P, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name CCKBR
Antigen Species Human
Immunogen C-FDGDSDSDSQSRVRNQ
Conjugation Un-conjugated
Alternate Names CCK-BR; CCK2-R; Cholecystokinin-2 receptor; CCK2R; CCK-B; Gastrin/cholecystokinin type B receptor; CCK-B receptor; GASR

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P5 - 6 µg/ml
WB1 - 3 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by antigen affinity chromatography.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12426 Mouse CCKBR

GeneID: 25706 Rat CCKBR

GeneID: 887 Human CCKBR

Background This gene encodes a G-protein coupled receptor for gastrin and cholecystokinin (CCK), regulatory peptides of the brain and gastrointestinal tract. This protein is a type B gastrin receptor, which has a high affinity for both sulfated and nonsulfated CCK analogs and is found principally in the central nervous system and the gastrointestinal tract. A misspliced transcript variant including an intron has been observed in cells from colorectal and pancreatic tumors. [provided by RefSeq, Jul 2008]
Research Area Metabolism antibody; Neuroscience antibody
Calculated MW 48 kDa

Images (3) Click the Picture to Zoom In

  • ARG63918 anti-CCKBR antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human uterus tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63918 anti-CCKBR antibody at 5 µg/ml dilution followed by AP-staining.

  • ARG63918 anti-CCKBR antibody WB image

    Western blot: 35 µg of Mouse pancreas (A) and Rat stomach (B) lysates (in RIPA buffer) stained with ARG63918 anti-CCKBR antibody at 1 µg/ml dilution and incubated at RT for 1 hour.

  • ARG63918 anti-CCKBR antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human pancreas tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0). The tissue section was stained with ARG63918 anti-CCKBR antibody at 6 µg/ml dilution followed by HRP-staining.