ARG62754

anti-CD18 / LFA1 beta antibody [IVA35]

Datasheet

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100 μg

399.00

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Overview

Product Description	Mouse Monoclonal antibody [IVA35] recognizes CD18 / LFA1 beta
Tested Reactivity	Bov
Tested Application	FACS, ICC/IF, IP
Specificity	The clone IVA35 reacts with CD18 antigen (bovine), a 90-95 kDa type I transmembrane protein (integrin) expressed on all leukocytes.
Host	Mouse
Clonality	Monoclonal
Clone	IVA35
Isotype	IgG1
Target Name	CD18 / LFA1 beta
Immunogen	Bovine leukocytes.
Conjugation	Un-conjugated
Alternate Names	MF17; LAD; CD antigen CD18; MFI7; MAC-1; Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; LCAMB; Integrin beta-2; Complement receptor C3 subunit beta; LFA-1; CD18

Application Instructions

Application Suggestion

Tested Application	Dilution
FACS	1 - 4 µg/ml
ICC/IF	Assay-dependent
IP	Assay-dependent

Application Note

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form	Liquid
Purification	Purified from ascites by protein-A affinity chromatography.
Purity	> 95% (by SDS-PAGE)
Buffer	PBS (pH 7.4) and 15 mM Sodium azide
Preservative	15 mM Sodium azide
Concentration	1 mg/ml
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 281877 Bovine ITGB2 Swiss-port # P32592 Bovine Integrin beta-2
Gene Symbol	ITGB2
Gene Full Name	integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
Background	This gene encodes an integrin beta chain, which combines with multiple different alpha chains to form different integrin heterodimers. Integrins are integral cell-surface proteins that participate in cell adhesion as well as cell-surface mediated signalling. The encoded protein plays an important role in immune response and defects in this gene cause leukocyte adhesion deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]
Function	Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation (By similarity). Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1. [UniProt]
Research Area	Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody
Calculated MW	85 kDa
PTM	Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters (PubMed:11700305). Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins (PubMed:11700305, PubMed:16301335).

Images (3) Click the Picture to Zoom In

ARG62754 anti-CD18 / LFA1 beta antibody [IVA35] ICC/IF image

Immunofluorescence: Acetone / methanol-fixed Bovine peripheral blood (lymphocyte-enriched suspension) stained with ARG62754 anti-CD18 / LFA1 beta antibody [IVA35] (green), DAPI (blue) for nuclear staining.
ARG62754 anti-CD18 / LFA1 beta antibody [IVA35] FACS image

Flow Cytometry: Separation of stained bovine lymphocytes (red-filled) from unstained bovine lymphocytes (black-dashed). Cells were stained with ARG62754 anti-CD18 / LFA1 beta antibody [IVA35] at 10 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
ARG62754 anti-CD18 / LFA1 beta antibody [IVA35] FACS image

Flow Cytometry: Bovine peripheral whole blood stained with ARG62754 anti-CD18 / LFA1 beta antibody [IVA35] at 10 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.