ARG63525
anti-CDC42 binding kinase alpha antibody
anti-CDC42 binding kinase alpha antibody for IHC-Formalin-fixed paraffin-embedded sections and Human
Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes CDC42 binding kinase alpha |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Cow, Dog |
| Tested Application | IHC-P |
| Specificity | This antibody is expected to recognise both reported Human isoforms (NP_055641.3 and NP_003598.2). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CDC42 binding kinase alpha |
| Antigen Species | Human |
| Immunogen | SGEVRLRQLEQFI-C |
| Conjugation | Un-conjugated |
| Alternate Names | DMPK-like alpha; CDC42-binding protein kinase alpha; PK428; Serine/threonine-protein kinase MRCK alpha; Myotonic dystrophy protein kinase-like alpha; MRCK alpha; EC 2.7.11.1; MRCKA; MRCK; Myotonic dystrophy kinase-related CDC42-binding kinase alpha |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by antigen affinity chromatography. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q5VT25 Human Serine/threonine-protein kinase MRCK alpha |
|---|---|
| Background | The protein encoded by this gene is a member of the Serine/Threonine protein kinase family. This kinase contains multiple functional domains. Its kinase domain is highly similar to that of the myotonic dystrophy protein kinase (DMPK). This kinase also contains a Rac interactive binding (CRIB) domain, and has been shown to bind CDC42. It may function as a CDC42 downstream effector mediating CDC42 induced peripheral actin formation, and promoting cytoskeletal reorganization. Multiple alternatively spliced transcript variants have been described, and the full-length nature of two of them has been reported. [provided by RefSeq, Jul 2008] |
| Research Area | Signaling Transduction antibody |
| Calculated MW | 197 kDa |
Images (2) Click the Picture to Zoom In
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ARG63525 anti-CDC42 binding kinase alpha antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human small intestine tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63525 anti-CDC42 binding kinase alpha antibody at 3.75 µg/ml dilution followed by AP-staining.
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ARG63525 anti-CDC42 binding kinase alpha antibody IHC-P image
Immunohistochemistry: Paraffin embedded Human Cerebellum (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63525 anti-CDC42 binding kinase alpha antibody at 3.8 µg/ml dilution followed by AP-staining.
