ARG57168
anti-CKB / Brain Creatine Kinase antibody [8E4]
anti-CKB / Brain Creatine Kinase antibody [8E4] for Flow cytometry,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [8E4] recognizes CKB / Brain Creatine Kinase |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 8E4 |
| Isotype | IgG2a, kappa |
| Target Name | CKB / Brain Creatine Kinase |
| Antigen Species | Human |
| Immunogen | Recombinant fragment around aa. 1-381 of Human CKB |
| Conjugation | Un-conjugated |
| Alternate Names | Creatine kinase B-type; EC 2.7.3.2; BCK; B-CK; CKBB; Creatine kinase B chain; HEL-S-29; HEL-211 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CKB |
| Gene Full Name | creatine kinase, brain |
| Background | The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized. [provided by RefSeq, Jul 2008] |
| Function | Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. [UniProt] |
| Calculated MW | 43 kDa |
Images (2) Click the Picture to Zoom In
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ARG57168 anti-CKB / Brain Creatine Kinase antibody [8E4] FACS image
Flow Cytometry: HeLa cell line stained with ARG57168 anti-CKB / Brain Creatine Kinase antibody [8E4] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody: Mouse IgG (black line).
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ARG57168 anti-CKB / Brain Creatine Kinase antibody [8E4] WB image
Western blot: 40 µg of 1) HeLa, 2) 293T, 3) K562, and 4) LnCap cell lysates stained with ARG57168 anti-CKB / Brain Creatine Kinase antibody [8E4] at 1:1000.
