ARG58426
anti-CKS2 antibody
anti-CKS2 antibody for Flow cytometry,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes CKS2 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CKS2 |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide corresponding to aa. 43-77 of Human CKS2. |
| Conjugation | Un-conjugated |
| Alternate Names | CKS-2; Cyclin-dependent kinases regulatory subunit 2; CKSHS2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | SH-SY5Y |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A and immunogen peptide. |
| Buffer | PBS and 0.09% (W/V) Sodium azide. |
| Preservative | 0.09% (W/V) Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P33552 Human Cyclin-dependent kinases regulatory subunit 2 |
|---|---|
| Gene Symbol | CKS2 |
| Gene Full Name | CDC28 protein kinase regulatory subunit 2 |
| Background | CKS2 protein binds to the catalytic subunit of the cyclin dependent kinases and is essential for their biological function. The CKS2 mRNA is found to be expressed in different patterns through the cell cycle in HeLa cells, which reflects specialized role for the encoded protein. [provided by RefSeq, Jul 2008] |
| Function | Binds to the catalytic subunit of the cyclin dependent kinases and is essential for their biological function. [UniProt] |
| Calculated MW | 10 kDa |
Images (2) Click the Picture to Zoom In
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ARG58426 anti-CKS2 antibody WB image
Western blot: 20 µg of SH-SY5Y cell lysate stained with ARG58426 anti-CKS2 antibody at 1:2000 dilution.
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ARG58426 anti-CKS2 antibody FACS image
Flow Cytometry: A549 cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% BSA to block non-specific protein-protein interactions and stained with ARG58426 anti-CKS2 antibody (right histogram) at 1:25 dilution for 60 min at 37°C, followed by incubation with DyLight® 488 labelled secondary antibody. Isotype control antibody (left histogram) was Rabbit IgG1 (1 µg/10^6 cells) used under the same conditions. Acquisition of >10000 events was performed.
