ARG64264
anti-CLIC4 antibody
anti-CLIC4 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes CLIC4 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Predict Reactivity | Dog, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CLIC4 |
| Antigen Species | Human |
| Immunogen | NGLKEEDKEPLIE-C |
| Conjugation | Un-conjugated |
| Alternate Names | Chloride intracellular channel protein 4; MTCLIC; H1; Intracellular chloride ion channel protein p64H1; huH1; p64H1; CLIC4L |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by antigen affinity chromatography. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9QYB1 Mouse Chloride intracellular channel protein 4 Swiss-port # Q9Y696 Human Chloride intracellular channel protein 4 |
|---|---|
| Background | Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 4 (CLIC4) protein, encoded by the CLIC4 gene, is a member of the p64 family; the gene is expressed in many tissues and exhibits a intracellular vesicular pattern in Panc-1 cells (pancreatic cancer cells). [provided by RefSeq, Jul 2008] |
| Research Area | Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 29 kDa |
Images (3) Click the Picture to Zoom In
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ARG64264 anti-CLIC4 antibody WB image
Western Blot: human kidney lysate (35 µg protein in RIPA buffer) stained with ARG64264 anti-CLIC4 antibody at 0.1 µg/ml dilution.
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ARG64264 anti-CLIC4 antibody ICC/IF image
Immunofluorescence: MIN6 cells transiently expressing Mouse Clic4. Cells were stained with ARG64264 anti-CLIC4 antibody at 5 µg/ml dilution. Hoechst (blue) for nuclear staining.
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ARG64264 anti-CLIC4 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human heart tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64264 anti-CLIC4 antibody at 5 µg/ml dilution followed by AP-staining.
