ARG66031
anti-CX3CL1 antibody
anti-CX3CL1 antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes CX3CL1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CX3CL1 |
| Antigen Species | Human |
| Immunogen | E. coli derived recombinant Human CX3CL1. (QHHGVTKCNI TCSKMTSKIP VALLIHYQQN QASCGKRAII LETRQHRLFC ADPKEQWVKD AMQHLDRQAA ALTRNG) |
| Conjugation | Un-conjugated |
| Alternate Names | neurotactin; CXC3; C3Xkine; NTN; NTT; C-X3-C motif chemokine 1; CXC3C; Neurotactin; ABCD-3; SCYD1; Fractalkine; Small-inducible cytokine D1; CX3C membrane-anchored chemokine; fractalkine |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.2) |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CX3CL1 |
| Gene Full Name | chemokine (C-X3-C motif) ligand 1 |
| Function | The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium. Binds to CX3CR1. [UniProt] |
| Calculated MW | 42 kDa |
| PTM | A soluble short 95 kDa form may be released by proteolytic cleavage from the long membrane-anchored form. O-glycosylated with core 1 or possibly core 8 glycans. |
Images (3) Click the Picture to Zoom In
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ARG66031 anti-CX3CL1 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human pancreas malignant ductal adenocarcinoma. The recommended ARG66031 anti-CX3CL1 antibody concentration is 0.25 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.
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ARG66031 anti-CX3CL1 antibody WB image
Western blot: 250 - 0.24 ng of Human Fractalkine stained with ARG66031 anti-CX3CL1 antibody, under non-reducing conditions.
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ARG66031 anti-CX3CL1 antibody WB image
Western blot: 250 - 0.24 ng of Human Fractalkine stained with ARG66031 anti-CX3CL1 antibody, under reducing conditions.
