ARG23716

anti-Citrulline antibody [6C2.1]

anti-Citrulline antibody [6C2.1] for ELISA,ICC/IF,Western blot and Other

Overview

Product Description Mouse Monoclonal antibody [6C2.1] recognizes Citrulline
Tested Reactivity Other
Tested Application ELISA, ICC/IF, WB
Specificity Specific for citrulline residues on modified proteins. Tested using citrulline conjugated Bovine Serum Albumin (BSA).
Host Mouse
Clonality Monoclonal
Clone 6C2.1
Isotype IgG1
Target Name Citrulline
Antigen Species Others
Immunogen Synthetic L-Citrulline conjugated to KLH.
Conjugation Un-conjugated

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA1:1000
ICC/IF1:50
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol.
Preservative 0.09% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Images (2) Click the Picture to Zoom In

  • ARG23716 anti-Citrulline antibody [6C2.1] ICC/IF image

    Immunofluorescence: HEK293 cells fixed with 5% Formaldehyde for 5 min. Cells were stained with ARG23716 anti-Citrulline antibody [6C2.1] (green) at 1:50 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) Primary antibodies. (D) Composite.

  • ARG23716 anti-Citrulline antibody [6C2.1] WB image

    Western blot: 0.5 µg of BSA, 2.0 µg of BSA, 0.5 µg of Citrulline-BSA and 2.0 µg of Citrulline-BSA stained with ARG23716 anti-Citrulline antibody [6C2.1] at 1:1000 for 2 hours at RT.