ARG56223
anti-Cyclophilin B antibody
anti-Cyclophilin B antibody for Flow cytometry,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Cyclophilin B |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Cyclophilin B |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide corresponding to aa. 161-195 of Human Cyclophilin B. |
| Conjugation | Un-conjugated |
| Alternate Names | HEL-S-39; CYPB; PPIase B; CYP-S1; Cyclophilin B; SCYLP; EC 5.2.1.8; S-cyclophilin; OI9; Rotamase B; Peptidyl-prolyl cis-trans isomerase B |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | HeLa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS and 0.09% (W/V) Sodium azide. |
| Preservative | 0.09% (W/V) Sodium azide. |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PPIB |
| Gene Full Name | peptidylprolyl isomerase B (cyclophilin B) |
| Background | The protein encoded by this gene is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum. It is associated with the secretory pathway and released in biological fluids. This protein can bind to cells derived from T- and B-lymphocytes, and may regulate cyclosporine A-mediated immunosuppression. Variants have been identified in this protein that give rise to recessive forms of osteogenesis imperfecta. [provided by RefSeq, Oct 2009] |
| Function | PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. [UniProt] |
| Calculated MW | 24 kDa |
Images (2) Click the Picture to Zoom In
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ARG56223 anti-Cyclophilin B antibody WB image
Western blot: 20 µg of HeLa cell lysate stained with ARG56223 anti-Cyclophilin B antibody at 1:2000 dilution.
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ARG56223 anti-Cyclophilin B antibody FACS image
Flow Cytometry: HeLa cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% BSA to block non-specific protein-protein interactions and stained with ARG56223 anti-Cyclophilin B antibody (right histogram) at 1:25 dilution for 60 min at 37°C, followed by incubation with DyLight® 488 labelled secondary antibody. Isotype control antibody (left histogram) was Rabbit IgG (1 µg/10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.
