ARG64036
anti-DSCAM antibody
anti-DSCAM antibody for Flow cytometry,ICC/IF,Western blot and Human
Neuroscience antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes DSCAM |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Dog |
| Tested Application | FACS, ICC/IF, WB |
| Specificity | This antibody is expected to recognise both reported isoforms (NP_001380.2 and NP_996770.1). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | DSCAM |
| Antigen Species | Human |
| Immunogen | C-DSWDSAQRTKDVSPQ |
| Conjugation | Un-conjugated |
| Alternate Names | CHD2-42; CHD2-52; CHD2; Down syndrome cell adhesion molecule |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by antigen affinity chromatography. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O60469 Human Down syndrome cell adhesion molecule |
|---|---|
| Background | This gene is a member of the immunoglobulin superfamily of cell adhesion molecules (Ig-CAMs), and is involved in human central and peripheral nervous system development. This gene is a candidate for Down syndrome and congenital heart disease (DSCHD). A gene encoding a similar Ig-CAM protein is located on chromosome 11. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Oct 2012] |
| Research Area | Neuroscience antibody |
| Calculated MW | 222 kDa |
| PTM | Phosphorylated at tyrosine residues. Phosphorylation is enhanced by netrin. |
Images (3) Click the Picture to Zoom In
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ARG64036 anti-DSCAM antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed MCF7 cells permeabilized with 0.15% Triton. Cells were stained with ARG64036 anti-DSCAM antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Phalloidin (red) for Actin filaments staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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ARG64036 anti-DSCAM antibody WB image
Western blot: 35 µg of HepG2 cell lysate (in RIPA buffer) stained with ARG64036 anti-DSCAM antibody at 2 µg/ml dilution and incubated at RT for 1 hour.
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ARG64036 anti-DSCAM antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed MCF7 cells permeabilized with 0.5% Triton. Cells were stained with ARG64036 anti-DSCAM antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
