ARG63462
anti-ELF1 antibody
anti-ELF1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Gene Regulation antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes ELF1 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | IHC-P, WB |
| Specificity | This antibody is expected to recognise both reported isoforms. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ELF1 |
| Antigen Species | Human |
| Immunogen | C-AMKQNELLEPNSF |
| Conjugation | Un-conjugated |
| Alternate Names | E74-like factor 1; ETS-related transcription factor Elf-1 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by antigen affinity chromatography. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P32519 Human ETS-related transcription factor Elf-1 Swiss-port # Q60775 Mouse ETS-related transcription factor Elf-1 |
|---|---|
| Background | This gene encodes an E26 transformation-specific related transcription factor. The encoded protein is primarily expressed in lymphoid cells and acts as both an enhancer and a repressor to regulate transcription of various genes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2009] |
| Research Area | Gene Regulation antibody |
| Calculated MW | 67 kDa |
Images (3) Click the Picture to Zoom In
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ARG63462 anti-ELF1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human thymus tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63462 anti-ELF1 antibody at 2.5 µg/ml dilution followed by AP-staining.
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ARG63462 anti-ELF1 antibody WB image
Western blot: 35 µg of Human lymph node (A), Human tonsil (B) and Mouse thymus (C) lysates (in RIPA buffer) stained with ARG63462 anti-ELF1 antibody at 0.3 µg/ml dilution and incubated at RT for 1 hour.
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ARG63462 anti-ELF1 antibody WB image
Western blot: 35 µg of Jurkat cell lysate (in RIPA buffer) stained with ARG63462 anti-ELF1 antibody at 0.1 µg/ml dilution and incubated at RT for 1 hour.
