ARG55693
anti-EN1 / Engrailed 1 antibody
anti-EN1 / Engrailed 1 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes EN1 / Engrailed 1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | EN1 / Engrailed 1 |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide corresponding to aa. 1-30 (N-terminus) of Human EN1 / Engrailed 1. |
| Conjugation | Un-conjugated |
| Alternate Names | Homeobox protein en-1; Homeobox protein engrailed-1; Hu-En-1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | NCI-H460 |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A and immunogen peptide. |
| Buffer | PBS and 0.09% (W/V) Sodium azide. |
| Preservative | 0.09% (W/V) Sodium azide. |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | EN1 |
| Gene Full Name | engrailed homeobox 1 |
| Background | Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. [provided by RefSeq, Jul 2008] |
| Cellular Localization | Nucleus. |
| Calculated MW | 40 kDa |
Images (3) Click the Picture to Zoom In
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ARG55693 anti-EN1 / Engrailed 1 antibody ICC/IF image
Immunofluorescence: U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then stained with ARG55693 anti-EN1 / Engrailed 1 antibody (green) at 1:25 dilution, 1 hour at 37°C. Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml, 1 hour at 37°C).
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ARG55693 anti-EN1 / Engrailed 1 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human lung carcinoma tissue stained with ARG55693 anti-EN1 / Engrailed 1 antibody.
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ARG55693 anti-EN1 / Engrailed 1 antibody WB image
Western blot: 20 µg of NCI-H460 whole cell lysate stained with ARG55693 anti-EN1 / Engrailed 1 antibody at 1:2000 dilution.
