ARG42641
anti-ENT1 antibody
anti-ENT1 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes ENT1 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Predict Reactivity | Rat |
| Tested Application | FACS, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ENT1 |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide between aa. 402-431 of Human ENT1. |
| Conjugation | Un-conjugated |
| Alternate Names | Solute carrier family 29 member 1; Equilibrative NBMPR-sensitive nucleoside transporter; Equilibrative nucleoside transporter 1; ENT1; Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter; Nucleoside transporter, es-type |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | Human breast and Mouse liver | ||||||||
| Observed Size | ~ 50 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A and immunogen peptide. |
| Buffer | PBS and 0.09% (W/V) Sodium azide. |
| Preservative | 0.09% (W/V) Sodium azide. |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q99808 Human Equilibrative nucleoside transporter 1 Swiss-port # Q9JIM1 Mouse Equilibrative nucleoside transporter 1 |
|---|---|
| Gene Symbol | SLC29A1 |
| Gene Full Name | solute carrier family 29 (equilibrative nucleoside transporter), member 1 |
| Background | This gene is a member of the equilibrative nucleoside transporter family. The gene encodes a transmembrane glycoprotein that localizes to the plasma and mitochondrial membranes and mediates the cellular uptake of nucleosides from the surrounding medium. The protein is categorized as an equilibrative (as opposed to concentrative) transporter that is sensitive to inhibition by nitrobenzylthioinosine (NBMPR). Nucleoside transporters are required for nucleotide synthesis in cells that lack de novo nucleoside synthesis pathways, and are also necessary for the uptake of cytotoxic nucleosides used for cancer and viral chemotherapies. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene. [provided by RefSeq, Jul 2008] |
| Function | Mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is sensitive (ES) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. It has a higher affinity for adenosine. Inhibited by dipyridamole and dilazep (anticancer chemotherapeutics drugs). [UniProt] |
| Cellular Localization | Basolateral cell membrane; Multi-pass membrane protein. Apical cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Note=Predominantly localized in the basolateral membrane in polarized MDCK cells. [UniProt] |
| Calculated MW | 50 kDa |
| PTM | Glycosylated. [UniProt] |
Images (4) Click the Picture to Zoom In
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ARG42641 anti-ENT1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human brain tissue stained with ARG42641 anti-ENT1 antibody at 1:500 dilution, for 1 hour at room temperature.
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ARG42641 anti-ENT1 antibody WB image
Western blot: 20 µg of Human breast lysate stained with ARG42641 anti-ENT1 antibody at 1:1000 dilution.
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ARG42641 anti-ENT1 antibody FACS image
Flow Cytometry: HepG2 cells were fixed with 2% paraformaldehyde 10 min. The cells were then incubated in 2% BSA to block non-specific protein-protein interactions followed by ARG42641 anti-ENT1 antibody (right histogram) at 1:25 dilution, for 60 min at 37°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (left histogram) was Rabbit IgG1 (1 µg/10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.
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ARG42641 anti-ENT1 antibody WB image
Western blot: 20 µg of Mouse liver lysate stained with ARG42641 anti-ENT1 antibody at 1:1000 dilution.
