ARG55364
anti-ETFA antibody
anti-ETFA antibody for ICC/IF,Western blot and Human,Mouse
Cancer antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes ETFA |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ETFA |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide corresponding to aa. 192-226 (Center) of Human ETFA. |
| Conjugation | Un-conjugated |
| Alternate Names | Electron transfer flavoprotein subunit alpha, mitochondrial; MADD; EMA; GA2; Alpha-ETF |
Application Instructions
| Application Suggestion |
|
||||||
|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | A431 |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A and immunogen peptide. |
| Buffer | PBS and 0.09% (W/V) Sodium azide |
| Preservative | 0.09% (W/V) Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P13804 Human Electron transfer flavoprotein subunit alpha, mitochondrial Swiss-port # Q99LC5 Mouse Electron transfer flavoprotein subunit alpha, mitochondrial |
|---|---|
| Gene Symbol | ETFA |
| Gene Full Name | electron-transfer-flavoprotein, alpha polypeptide |
| Background | ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Function | The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase). [UniProt] |
| Cellular Localization | Mitochondrion matrix. |
| Research Area | Cancer antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 35 kDa |
| PTM | The N-terminus is blocked. |
Images (2) Click the Picture to Zoom In
-
ARG55364 anti-ETFA antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG55364 anti-ETFA antibody (green) at 1:25 dilution. Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red). DAPI (blue) for nuclear staining.
-
ARG55364 anti-ETFA antibody WB image
Western blot: 35 µg of A431 cell lysate stained with ARG55364 anti-ETFA antibody at 1:1000 dilution.
