ARG56850

anti-GPAT1 antibody

anti-GPAT1 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes GPAT1
Tested Reactivity Ms, Rat
Predict Reactivity Hu
Tested Application ICC/IF, IHC-P, WB
Specificity At least four isoforms of GPAT1 are known to exist. This antibody will detect only the two largest isoforms.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name GPAT1
Antigen Species Human
Immunogen Synthetic peptide (15 aa) within aa. 730-780 of the Human GPAT1.
Conjugation Un-conjugated
Alternate Names GPAT; GPAT-1; Glycerol-3-phosphate acyltransferase 1, mitochondrial; GPAT1; EC 2.3.1.15

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF20 µg/ml
IHC-P2.5 µg/ml
WB1 - 2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Rat brain tissue lysate

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS and 0.02% Sodium azide.
Preservative 0.02% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 14732 Mouse GPAM

GeneID: 29653 Rat GPAM

Swiss-port # P97564 Rat Glycerol-3-phosphate acyltransferase 1, mitochondrial

Swiss-port # Q61586 Mouse Glycerol-3-phosphate acyltransferase 1, mitochondrial

Gene Symbol GPAM
Gene Full Name glycerol-3-phosphate acyltransferase, mitochondrial
Background This gene encodes a mitochondrial enzyme which prefers saturated fatty acids as its substrate for the synthesis of glycerolipids. This metabolic pathway's first step is catalyzed by the encoded enzyme. Two forms for this enzyme exist, one in the mitochondria and one in the endoplasmic reticulum. Two alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Oct 2011]
Function Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipid biosynthesis. [UniProt]
Calculated MW 94 kDa

Images (4) Click the Picture to Zoom In

  • ARG56850 anti-GPAT1 antibody IHC-P image

    Immunohistochemistry: Rat Brain tissue stained with ARG56850 anti-GPAT1 antibody at 20 µg/ml dilution.

  • ARG56850 anti-GPAT1 antibody WB image

    Western blot: Rat brain tissue lysate stained with ARG56850 anti-GPAT1 antibody at (A) 1 and (B) 2 µg/ml dilution.

  • ARG56850 anti-GPAT1 antibody IHC-P image

    Immunohistochemistry: Rat brain stained with ARG56850 anti-GPAT1 antibody at 2.5 µg/ml dilution.

  • ARG56850 anti-GPAT1 antibody IHC-P image

    Immunohistochemistry: Mouse brain tissue stained with ARG56850 anti-GPAT1 antibody at 5 µg/ml dilution.