ARG59488
anti-MAF1 antibody
anti-MAF1 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes MAF1 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Predict Reactivity | Bov, Rat |
| Tested Application | FACS, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | MAF1 |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide corresponding to aa. 90-117 of Human MAF1. |
| Conjugation | Un-conjugated |
| Alternate Names | Repressor of RNA polymerase III transcription MAF1 homolog |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | Neuro-2a |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A and immunogen peptide. |
| Buffer | PBS and 0.09% (W/V) Sodium azide. |
| Preservative | 0.09% (W/V) Sodium azide. |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9D0U6 Mouse Repressor of RNA polymerase III transcription MAF1 homolog Swiss-port # Q9H063 Human Repressor of RNA polymerase III transcription MAF1 homolog |
|---|---|
| Gene Symbol | MAF1 |
| Gene Full Name | MAF1 homolog (S. cerevisiae) |
| Background | This gene encodes a protein that is similar to Maf1, a Saccharomyces cerevisiae protein highly conserved in eukaryotic cells. Yeast Maf1 is a negative effector of RNA polymerase III (Pol III). It responds to changes in the cellular environment and represses pol III transcription. Biochemical studies identified the initiation factor TFIIIB as a target for Maf1-dependent repression. [provided by RefSeq, Jul 2008] |
| Function | Element of the mTORC1 signaling pathway that acts as a mediator of diverse signals and that represses RNA polymerase III transcription. Inhibits the de novo assembly of TFIIIB onto DNA. [UniProt] |
| Cellular Localization | Nucleus. Cytoplasm. [UniProt] |
| Calculated MW | 29 kDa |
| PTM | Phosphorylated at Ser-60, Ser-68 and Ser-75; the major sites of phosphorylation. Nuclear accumulation correlates with a concomitant dephosphorylation. Phosphorylation may attenuate its RNA polymerase III-repressive function. Sumoylated with SUMO1 and SUMO2, mainly on Lys-35. Desumoylated by SENP1. SUMOylation promotes the ability of MAF1 to repress transcription and suppress colony formation. [UniProt] |
Images (3) Click the Picture to Zoom In
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ARG59488 anti-MAF1 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human lung carcinoma stained with ARG59488 anti-MAF1 antibody.
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ARG59488 anti-MAF1 antibody WB image
Western blot: 35 µg of Neuro-2a cell lysate stained with ARG59488 anti-MAF1 antibody.
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ARG59488 anti-MAF1 antibody FACS image
Flow Cytometry: Neuro-2a cells stained with ARG59488 anti-MAF1 antibody (right histogram) or without primary antibody as control (left histogram), followed by FITC labelled secondary antibody.
