ARG63842
anti-NHERF2 antibody
anti-NHERF2 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes NHERF2 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P, WB |
| Specificity | Please note that this variant has an extra insertion of 11 residues compared to NP_004776.3. The immunizing peptide represents this insertion. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | NHERF2 |
| Antigen Species | Human |
| Immunogen | C-DGSAWKQDPFQ |
| Conjugation | Un-conjugated |
| Alternate Names | Tyrosine kinase activator protein 1; NHE3RF2; E3KARP; Na; +; OCTS2; NHERF-2; Solute carrier family 9 isoform A3 regulatory factor 2; Sodium-hydrogen exchanger regulatory factor 2; NHE3 kinase A regulatory protein E3KARP; NHERF2; SIP-1; SIP1; TKA-1; SRY-interacting protein 1 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Microwaved tissue section in Tris/EDTA buffer (pH 9.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by antigen affinity chromatography. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q15599 Human Na(+)/H(+) exchange regulatory cofactor NHE-RF2 |
|---|---|
| Background | This gene encodes a member of the NHERF family of PDZ scaffolding proteins. These proteins mediate many cellular processes by binding to and regulating the membrane expression and protein-protein interactions of membrane receptors and transport proteins. The encoded protein plays a role in intestinal sodium absorption by regulating the activity of the sodium/hydrogen exchanger 3, and may also regulate the cystic fibrosis transmembrane regulator (CFTR) ion channel. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011] |
| Research Area | Signaling Transduction antibody |
| Calculated MW | 37 kDa |
Images (2) Click the Picture to Zoom In
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ARG63842 anti-NHERF2 antibody WB image
Western Blot: human kidney lysate (35 µg protein in RIPA buffer) stained with ARG63842 anti-NHERF2 (isoform a) antibody at 0.3 µg/ml dilution.
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ARG63842 anti-NHERF2 antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Kidney. (Microwaved antigen retrieval with Tris/EDTA buffer pH9) stained with ARG63842 anti-NHERF2 (isoform a) antibody at 3 µg/ml dilution followed by HRP-staining.
