ARG43949

anti-PPM1N antibody

anti-PPM1N antibody for ELISA,Flow cytometry,ICC/IF,Western blot and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes PPM1N
Tested Reactivity Hu
Tested Application ELISA, FACS, ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name PPM1N
Antigen Species Human
Immunogen Human PPM1N recombinant protein
Expression System E.coli
Conjugation Un-conjugated
Protein Full Name Probable protein phosphatase 1N
Alternate Names PPM1N; Protein Phosphatase, Mg2+/Mn2+ Dependent 1N (Putative); Probable Protein Phosphatase 1N; FLJ40125; Protein Phosphatase, Mg2+/Mn2+ Dependent, 1N (Putative); EC 3.1.3.16

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA0.1-0.5 µg/ml
FACS1-3 µg /1x10^6 cells
ICC/IF5 µg/ml
WB0.25-0.5 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purified with Immunogen.
Buffer 0.9% NaCl, 0.2% Na2HPO4 and 4% Trehalose.
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 147699 Human PPM1N

Swiss-port # Q8N819 Human Probable protein phosphatase 1N

Gene Symbol PPM1N
Gene Full Name Protein Phosphatase, Mg2+/Mn2+ Dependent 1N (Putative)
Background Predicted to enable metal ion binding activity and protein serine/threonine phosphatase activity. Predicted to be involved in negative regulation of I-kappaB kinase/NF-kappaB signaling and positive regulation of canonical Wnt signaling pathway. Predicted to be active in cytosol and nucleus.
Function H2O + O-phospho-L-seryl-[protein] = L-seryl-[protein] + phosphate
Cellular Localization Cytosol, Nucleus
Calculated MW 46 kDa

Images (3) Click the Picture to Zoom In

  • ARG43949 anti-PPM1N antibody ICC/IF image

    Immunofluorescence: A549 cells stained with ARG43949 anti-PPM1N antibody at 5 μg/ml dilution.

  • ARG43949 anti-PPM1N antibody WB image

    Western blot: Hela, K562 and A549 stained with ARG43949 anti-PPM1N antibody at 0.5 μg/mL dilution.

  • ARG43949 anti-PPM1N antibody FACS image

    Flow Cytometry: MCF-7 cells stained with ARG43949 anti-PPM1N antibody (blue) at 1 μg/1x10^6 cells dilution.