ARG42482
anti-PYCR1 antibody
anti-PYCR1 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes PYCR1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PYCR1 |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide between aa. 291-319 of Human PYCR1. |
| Conjugation | Un-conjugated |
| Alternate Names | PIG45; EC 1.5.1.2; P5CR 1; Pyrroline-5-carboxylate reductase 1, mitochondrial; PRO3; P5C reductase 1; P5C; ARCL3B; P5CR; ARCL2B; PP222; PYCR |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | WiDr | ||||||||
| Observed Size | ~ 33 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A and immunogen peptide. |
| Buffer | PBS and 0.09% (W/V) Sodium azide. |
| Preservative | 0.09% (W/V) Sodium azide. |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P32322 Human Pyrroline-5-carboxylate reductase 1, mitochondrial |
|---|---|
| Gene Symbol | PYCR1 |
| Gene Full Name | pyrroline-5-carboxylate reductase 1 |
| Background | This gene encodes an enzyme that catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP(+) in some cell types. The protein forms a homopolymer and localizes to the mitochondrion. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013] |
| Function | Housekeeping enzyme that catalyzes the last step in proline biosynthesis. Can utilize both NAD and NADP, but has higher affinity for NAD. Involved in the cellular response to oxidative stress. [UniProt] |
| Cellular Localization | Mitochondrion. [UniProt] |
| Calculated MW | 33 kDa |
Images (3) Click the Picture to Zoom In
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ARG42482 anti-PYCR1 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human colon carcinoma tissue stained with ARG42482 anti-PYCR1 antibody.
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ARG42482 anti-PYCR1 antibody WB image
Western blot: 35 µg of WiDr cell lysate stained with ARG42482 anti-PYCR1 antibody.
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ARG42482 anti-PYCR1 antibody FACS image
Flow Cytometry: HepG2 cells stained with ARG42482 anti-PYCR1 antibody (right histogram) or without primary antibody as control (left histogram), followed by incubation with FITC labelled secondary antibody.
