ARG40042
anti-RPLP0 antibody
anti-RPLP0 antibody for ICC/IF,Western blot and Human,Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes RPLP0 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | RPLP0 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1-317 of Human RPLP0 (NP_000993.1). |
| Conjugation | Un-conjugated |
| Alternate Names | P0; L10E; 60S acidic ribosomal protein P0; LP0; RPP0; 60S ribosomal protein L10E; PRLP0 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | Mouse thymus and OVCAR3 | ||||||
| Observed Size | 37 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | RPLP0 |
| Gene Full Name | ribosomal protein, large, P0 |
| Background | Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein, which is the functional equivalent of the E. coli L10 ribosomal protein, belongs to the L10P family of ribosomal proteins. It is a neutral phosphoprotein with a C-terminal end that is nearly identical to the C-terminal ends of the acidic ribosomal phosphoproteins P1 and P2. The P0 protein can interact with P1 and P2 to form a pentameric complex consisting of P1 and P2 dimers, and a P0 monomer. The protein is located in the cytoplasm. Transcript variants derived from alternative splicing exist; they encode the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008] |
| Function | Ribosomal protein P0 is the functional equivalent of E.coli protein L10. [UniProt] |
| Cellular Localization | Nucleus. Cytoplasm. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs (PubMed:19188445, PubMed:17289661). [UniProt] |
| Calculated MW | 34 kDa |
Images (2) Click the Picture to Zoom In
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ARG40042 anti-RPLP0 antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG40042 anti-RPLP0 antibody.
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ARG40042 anti-RPLP0 antibody WB image
Western blot: 25 µg of Mouse thymus and OVCAR3 cell lysates stained with ARG40042 anti-RPLP0 antibody at 1:1000 dilution.
