ARG42178
anti-RTCB / HSPC117 antibody
anti-RTCB / HSPC117 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Goat Polyclonal antibody recognizes RTCB / HSPC117 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Cow, Dog, Pig |
| Tested Application | IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | RTCB / HSPC117 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around the internal region of Human RTCB / HSPC117. (C-QADPNKVSARAKKR) (NP_055121.1) |
| Conjugation | Un-conjugated |
| Alternate Names | EC 6.5.1.3; HSPC117; DJ149A16.6; C22orf28; tRNA-splicing ligase RtcB homolog; FAAP |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | HeLa | ||||||
| Observed Size | ~ 55 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | RTCB |
| Gene Full Name | RNA 2',3'-cyclic phosphate and 5'-OH ligase |
| Function | Catalytic subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'-phosphodiester. May act as an RNA ligase with broad substrate specificity, and may function toward other RNAs. [UniProt] |
| Cellular Localization | Nucleus. Cytoplasm. Note=Enters into the nucleus in case of active transcription while it accumulates in cytosol when transcription level is low. [UniProt] |
| Calculated MW | 55 kDa |
Images (2) Click the Picture to Zoom In
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ARG42178 anti-RTCB / HSPC117 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human testis tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG42178 anti-RTCB / HSPC117 antibody at 5 µg/ml dilution followed by AP-staining.
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ARG42178 anti-RTCB / HSPC117 antibody WB image
Western blot: 35 µg of HeLa cell lysate (in RIPA buffer) stained with ARG42178 anti-RTCB / HSPC117 antibody at 0.3 µg/ml dilution and incubated at RT for 1 hour.
