ARG57108
anti-Ras (pan) antibody [2G9]
anti-Ras (pan) antibody [2G9] for Flow cytometry,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [2G9] recognizes Ras (pan) |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 2G9 |
| Isotype | IgG2a, kappa |
| Target Name | Ras (pan) |
| Antigen Species | Human |
| Immunogen | Recombinant fragment around aa. 1-186 of Human Ras (pan) |
| Conjugation | Un-conjugated |
| Alternate Names | GTPase NRas; N-ras; NRAS1; CMNS; Transforming protein N-Ras; NCMS; ALPS4; NS6 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NRAS |
| Gene Full Name | neuroblastoma RAS viral (v-ras) oncogene homolog |
| Background | This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia. [provided by RefSeq, Jun 2011] |
| Function | Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. [UniProt] |
| Calculated MW | 21 kDa |
| PTM | Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi. Acetylation at Lys-104 prevents interaction with guanine nucleotide exchange factors (GEFs). |
Images (3) Click the Picture to Zoom In
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ARG57108 anti-Ras (pan) antibody [2G9] FACS image
Flow Cytometry: HeLa cell line stained with ARG57108 anti-Ras (pan) antibody [2G9] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody: Mouse IgG (black line).
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ARG57108 anti-Ras (pan) antibody [2G9] WB image
Western blot: 35 µg of 293T cell lysates stained with ARG57108 anti-Ras (pan) antibody [2G9] at 1) 1:500, 2) 1:1000, and 3) 1:5000.
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ARG57108 anti-Ras (pan) antibody [2G9] WB image
Western blot: 200 ng of NRAS, KRAS and HRAS recombinant proteins stained with ARG57108 anti-Ras (pan) antibody [2G9].
