ARG11154
anti-SARS-CoV / SARS-CoV-2 Spike protein (RBD) antibody
anti-SARS-CoV / SARS-CoV-2 Spike protein (RBD) antibody for ICC/IF,Western blot and Virus
Overview
| Product Description | Rabbit Polyclonal antibody recognizes SARS-CoV / SARS-CoV-2 Spike protein (RBD) |
|---|---|
| Tested Reactivity | Virus |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | SARS-CoV / SARS-CoV-2 Spike protein (RBD) |
| Antigen Species | Virus |
| Immunogen | Recombinant SARS-CoV-2 Spike protein ACE2 binding domain (aa. 308-541 in the Spike protein sequence in Wuhan-Hu-1). |
| Conjugation | Un-conjugated |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified |
| Buffer | PBS, 5 mM Sodium azide and 50% Glycerol. |
| Preservative | 5 mM Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Images (4) Click the Picture to Zoom In
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ARG11154 anti-SARS-CoV / SARS-CoV-2 Spike protein (RBD) antibody ICC/IF image
Immunofluorescence: HEK293 cells transfected with pCI-Neo-Mod vector including DNA encoding the SARS-CoV-2 Spike protein ACE2 binding domain (aa. 308-541 in the Spike protein sequence in Wuhan-Hu-1). This region was used as the immunogen for development of ARG11154 anti-SARS-CoV / SARS-CoV-2 Spike protein (RBD) antibody. HEK293 cells stained with ARG11154 (red) at 1:3000 dilution, and co-stained with anti-beta Tubulin antibody (green) at 1:5000 dilution. Hoechst (blue) for nuclear staining.
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ARG11154 anti-SARS-CoV / SARS-CoV-2 Spike protein (RBD) antibody WB image
Western blot: 1) Non-transfected HEK293 cells, 2) HEK293 cells transfected with pCI-Neo-Mod vector containing the SARS-CoV-2 Spike protein ACE2 binding domain cDNA, and 3) HEK293 cells transfected with pCI-Neo-GFP vector containing the SARS-CoV-2 Spike protein ACE2 binding domain cDNA. Cell lysates were stained with ARG11154 anti-SARS-CoV / SARS-CoV-2 Spike protein (RBD) antibody (green) at 1:2000 dilution. The same blot was simultaneously stained with anti-beta Tubulin antibody (red) at 1:5000 dilution.
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ARG11154 anti-SARS-CoV / SARS-CoV-2 Spike protein (RBD) antibody ICC/IF image
Immunofluorescence: Vero cells infected with SARS-CoV-2 virus. Cells were stained with ARG11154 anti-SARS-CoV / SARS-CoV-2 Spike protein (RBD) antibody (red). DAPI (blue) for nuclear staining.
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ARG11154 anti-SARS-CoV / SARS-CoV-2 Spike protein (RBD) antibody WB image
Western blot: Full-length recombinant SARS-CoV-2 Spike protein expressed in HEK293 cells. Loading of 1 µg (left) or 100 ng (right). The blots were stained with ARG11154 anti-SARS-CoV / SARS-CoV-2 Spike protein (RBD) antibody.
