ARG64276

anti-SMEK1 / KIAA2010 antibody

anti-SMEK1 / KIAA2010 antibody for Western blot and Human

Gene Regulation antibody

Overview

Product Description Goat Polyclonal antibody recognizes SMEK1 / KIAA2010
Tested Reactivity Hu
Predict Reactivity Ms, Rat, Dog
Tested Application WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name SMEK1 / KIAA2010
Antigen Species Human
Immunogen C-EQQRERQDNPKLD
Conjugation Un-conjugated
Alternate Names FLFL1; SMEK homolog 1; PP4R3A; smk1; Serine/threonine-protein phosphatase 4 regulatory subunit 3A; SMEK1; MSTP033; smk-1; PP4R3; KIAA2010

Application Instructions

Application Suggestion
Tested Application Dilution
WB0.3 - 1 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by antigen affinity chromatography.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 55671 Human PPP4R3A

Swiss-port # Q6IN85 Human Serine/threonine-protein phosphatase 4 regulatory subunit 3A

Gene Symbol SMEK1
Gene Full Name SMEK homolog 1, suppressor of mek1 (Dictyostelium)
Function Regulatory subunit of serine/threonine-protein phosphatase 4. May regulate the activity of PPP4C at centrosomal microtubule organizing centers. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on 'Ser-140' (gamma-H2AFX) generated during DNA replication and required for DNA DSB repair. [UniProt]
Research Area Gene Regulation antibody
Calculated MW 95 kDa

Images (2) Click the Picture to Zoom In

  • ARG64276 anti-SMEK1 / KIAA2010 antibody WB image

    Western Blot: Human Pancreas lysate (35 µg protein in RIPA buffer) stained with ARG64276 anti-SMEK1 / KIAA2010 antibody at 0.3 µg/ml dilution.

  • ARG64276 anti-SMEK1 / KIAA2010 antibody WB image

    Western Blot: 1). Mock transfection; 2) SMEK1 (RC221040) expressing plasmid transfected HEK293 cell lysate standed with ARG64276 anti-SMEK1 / KIAA2010 antibody